山东地区乙型肝炎病毒基因分型的研究  

The distribution of hepatitis virus genotypesin Shandong province

作　　者：孙正伟[1] 王威[1] 董振芳[1] 孙向阳[1] 

机构地区：[1]山东省立医院,山东济南250021

出　　处：《山东医药》2005年第31期3-4,共2页Shandong Medical Journal

基　　金：山东省卫生厅卫生科技发展计划(2001CA1DBB1)

摘　　要：目的了解山东地区慢性乙型肝炎患者HBV基因分布特征,探讨其基因型与病毒复制的关系。方法采用PCR、微板核酸分子杂交和酶联显色技术,对该地区166例慢性乙型肝炎患者血清HBV-DNA进行了基因分型。结果166例患者中C基因型115例(115/131),分型率为87.79%;D基因型16例(16/131),分型率为12.21%。未发现A、B、E和F基因型。结论山东地区HBV基因型为C型和D型,优势基因型为C型。Objective： To ascertain the distribution of HBV genotypes in Shandong province and to observe the relationships between the HBV genotypes and it＇s reproducing. Methods： PCR, ELISA, and Micro-plate nucleic acid hybridization were used to determine HBV genotypes. Results： 166 hepatitis B patients were studied. 87.79% （115/131） was genotype C and 12.21% （16/131） was genotype D. Genotype A, B, E and F were not detected. Conclusion： HBV genotype C is predominant in Shandong province, and genotype D is also discovered.

关 键 词：肝炎病毒 基因型 核酸杂交 

分 类 号：R575.1[医药卫生—消化系统]