反相高效液相色谱法检测辣椒酱中的苏丹红染料  

DETERMINATION OF RED DYESTUFFS OF SUDAN IN THE CHILLI SAUCE BY INVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：梁景波 黄锦燕 

机构地区：[1]淄博市环境监测站,淄博255040 [2]淄博市食品安全检测中心,淄博255041

出　　处：《化学分析计量》2005年第5期27-29,共3页Chemical Analysis And Meterage

摘　　要：采用反相高效液相色谱法检测辣椒酱中的苏丹红染料,辣椒酱经正己烷-丙酮溶剂提取,用氧化铝层析柱进行固相萃取净化,用紫外可见光检测器检测,选择ZorbaxSB-C18色谱柱检测苏丹红I、苏丹红Ⅱ、苏丹红Ⅲ和苏丹红Ⅳ,加标回收率为91%～95%,测定结果的相对标准偏差为0.89%～1.79%.Determination of red dyestuffs of Sudan in the chilli sauce by inversed phase high performance liquid chromatography was introduced. Chilli sauce sample was extracted with n-hexane - acetone solvent, cleaned up with aluminum oxide layer chromatography column and detected with visible ultraviolet detector. ZorbaxSB- C18chromatograph column was selected to detect Sudan red Ⅰ , Sudan red Ⅱ , Sudan red Ⅲ and Sudan red Ⅳ. The recoveries were 91% -95% , the relative standard deviations of the determining results were 0.89% - 1.79%.

关 键 词：高效液相色谱法检 测 辣椒酱 苏丹红 反相高效液相色谱法 光检测器 苏丹 染料 相对标准偏差 加标回收率 

分 类 号：O657.72[理学—分析化学] X789.03[理学—化学]