人外周血单个核细胞对人牙周膜成纤维细胞增殖和分化的影响  

The effects of human peripheral blood mononuclear cells on the proliferation and differentiation of human periodontal ligament fibroblast cells

作　　者：王燕[1] 丁寅[1] 李永明[1] 董蕊[1] 王文清[2] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032 [2]第四军医大学西京医院血液科

出　　处：《临床口腔医学杂志》2005年第10期610-612,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助项目(30300393)

摘　　要：目的:观察人外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)对人牙周膜成纤维细胞(human periodontal ligament fibroblast cells,HPLFs)增殖分化的影响,为进一步探讨正畸牙齿移动的生物学机制奠定基础。方法:建立人PBMCs与HPLFs共培养系统,通过细胞计数及生化检测法观察人外周血单个核细胞对人牙周膜成纤维细胞增殖和分化的影响。结果:3 d和5 d时,共培养组HPLFs细胞计数分别为4.5×104及8.5×104,明显高于对照组,且两组分别与对照组HPLFs细胞计数有显著性差异(P<0.05)。transwell共培养组与对照组相比,在3 d时,两组HPLFs分泌型ALP活性有显著性差异(P<0.05),5 d及7 d时差异尤其显著(P<0.01),tran-swell共培养组HPLFs分泌型ALP活性低于对照组。结论:人PBMCs能促进HPLFs的增殖,但抑制HPLFs分泌型ALP活性。Objective： In order to determine the effect of factors pertaining to the development and metabolism of periodontal ligament, the author investigated the effect of human peripheral blcood mononuclear cells （PBMCs） on human periodontal ligament fibroblast cells （HPLFs）. Method： The co-culture system of HPLFs and human PBMCs separated by transwell was established. The proliferation and alkaline phosphatase （ALPase） activity of HPLFs were evaluated by cell number counter and enzyme kinetics methods after 1, 3, 5, 7 days. Result：After co-culture, the number of HPLFs were 4.5×10^4 after 3 days and 8.5×10^4 after 5 days . Compared with the control group, the difference between the two groups and control group was significant （P〈0.05 ）. The ALPase activities of HPLFs were reduced by co-culture with human PBMCs after 3, 5, 7 days. Conclusion： human PBMCs could induce the proliferation of HPLFs, while restrain the differentiation of HPLFs.

关 键 词：人外周血单个核细胞 牙周膜成纤维细胞 增殖 碱性磷酸酶活性 

分 类 号：R780.2[医药卫生—口腔医学]