茶叶中维生素C的荧光测定法  

Spectrofluortmetry determination for Vitamin C in Tea

作　　者：郑玉聪[1] 谢狄霖[1] 张维镇[2] 

机构地区：[1]福建省医学科学研究所,福州350001 [2]福建医科大学

出　　处：《福建分析测试》2005年第4期2307-2308,2317,共3页Fujian Analysis & Testing

摘　　要：目的建立荧光分光光度法测定茶叶中维生素C的含量。方法茶叶经草酸提取,活性炭氧化后,与邻苯二胺反应,在激发波长355nm,发射波长425nm测定荧光强度。结果维生素C在2-20mg/L浓度范围内呈现良好的线性关系,相关系数为0.9996。平均回收率为99.5%。结论采用荧光法测定茶叶中维生素C含量,具有准确、简便、快速、灵敏等优点。Objective to establish a spectrofluortmetry method for determination of Vitamin C in Tea. Method the tea was extracted by oxalic acid, actived by carbon oxidation, and reacdved by O -phenylenediamine, The fluorescence intersity has been measured at excitated wavelength 355nm and emission wavelength 425nm. Results The linear range of the vitamin C was 2 -20 mg/L, relationship coefficient was 0. 9996. The average recovery rate was 99.5%. Conclusion it is accurate, simple, rapid, sensitive to determined the vitamin C in tea with the fluorescence method.

关 键 词：荧光分光光度法 茶叶 维生素C 维生素C含量 荧光测定法 分光光度法测定 平均回收率 荧光法测定 邻苯二胺 激发波长 

分 类 号：TQ455.5[化学工程—农药化工] TS215[轻工技术与工程—粮食、油脂及植物蛋白工程]