β模块配型技术的建立  

Establishment of β Block Matching Technique

作　　者：朱发明[1] 吕沁风[1] 章伟[1] 张海琴[1] 傅启华[1] 严力行[1] 

机构地区：[1]浙江省血液中心,杭州310006

出　　处：《中国实验血液学杂志》2005年第5期901-903,共3页Journal of Experimental Hematology

基　　金：浙江省医药卫生科学研究基金资助项目;编号2003B026

摘　　要：本研究目的是建立β模块配型技术。采用盐析法从全血中抽提DNA,采用PCR和GeneScan技术进行β模块配型。结果表明:①在检测的100例样本中,扩增出的DNA片段长度不一,其范围为91-197bp;共形成4个相对较集中的区域,分别为91-93bp、105-113bp、125-139bp、177-197bp,其中所有样本均存在91bp的片段。②在样本中不同长度的DNA片段的个数呈现高度多态性,其范围为7-24个。结论:建立的β模块配型技术是可行的,可用于造血干细胞移植供者的选择。The purpose of this study was to establish β block matching technique. DNA was extracted from whole blood by salting-out method, β block matching was performed by PCR and GeneScan technique. The results showed that the length of fragments amplificated in 100 samples was different and the range of them was 91 - 197 bp. Amplification fragments could be divided into four regions： 91 -93, 105 -113, 125 -139 and 177 - 197 bp respectively, 91 bp DNA fragments could be found in all of samples. The numbers of DNA fragments with different length have been shown high polymorphism and they focused on the range of seven to twenty four. In conclusion, the β block matching technique is reliable and applicable to the selection of hematopoietic stem cell transplantation donors.

关 键 词：β模块配型 造血干细胞移植 基因扫描 

分 类 号：R457.7[医药卫生—治疗学]