酶的定向进化及其应用  

Methods and application of directed enzyme evolution

作　　者：孙志浩[1] 柳志强[1] 

机构地区：[1]江南大学生物工程学院生物催化研究室工业生物技术教育部重点实验室,无锡214036

出　　处：《生物加工过程》2005年第3期7-13,共7页Chinese Journal of Bioprocess Engineering

基　　金：国家973项目(2003CB716008);国家自然科学基金项目(20476039)

摘　　要：综述了生物催化剂(酶)分子定向进化的产生、原理、方法及应用,深入阐述酶分子定向进化过程中的相关问题,重点介绍了易错PCR(Error-prone PCR)和DNA改组(DNA shuffling)等几种典型的酶定向进化方法与成功实例,展望了生物定向进化研究的发展前景。The history, principle, methods and applications of directed enzyme evolution are reviewed in this article, and the questions met in the procedure of directed enzyme evolution are further discussed. Several representative methods, such as error prone PCR and DNA shuffling, and successful samples are introduced. Meanwhile, the development of directed enzyme evolution is also prospected,

关 键 词：生物催化剂 蛋白质工程 非理性设计 定向进化 易错PCR DNA改组 

分 类 号：X17[环境科学与工程—环境科学]