不对称还原2-辛酮的菌株筛选和反应条件的研究  

Screening and optimization of asymmetric reduction of 2-octanone by Oenococcus oeni CECT 4730

作　　者：胡健[1] 徐岩 

机构地区：[1]江南大学生物工程学院教育部工业生物技术重点实验室,无锡214036

出　　处：《生物加工过程》2005年第3期42-46,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(编号:20376031)

摘　　要：从本实验室保藏菌株中筛选出一株酒明串珠菌Oenococcus oeniCECT 4730,能不对称还原2-辛酮得到R-2-辛醇。对反应条件的研究表明:Tris-硼酸(TBE)是最佳缓冲液,葡萄糖是最佳辅助底物,壬烷是最佳有机相,当采用300 mmol/L的TBE缓冲液3、00 mmol/L的葡萄糖、1∶1(体积比)的两相比1、50 g/L的湿菌体质量浓度下,可以实现3%的2-辛酮转化为R-2-辛醇(转化率>99%),并且e.e.值为97%。Asymmetry reductions of 2-octanone by yeast, mold and bacteria in aqueous-organic solvent biphasic systems were investigated. The Oenococcus oeni CECT 4730 catalyzed the reduction of 2-octanone to R-2-octanol with higher activity and optical purity. The conditions for whole cell reaction were investigated. The suitable buffer was Tris-Borate （TBE）, the optimum co-substrate was glucose, the best organic solvent was n-nonane. When the concentration of 2-octanone was 234 mmol/L, with 300 mmol/L TBE and 300 mmol/L glucose （aqueous phase/organic phase = 1：1 ）, the conversion and enantiomeric excess were 99% and 97 %.

关 键 词：不对称还原 2-辛醇 菌株筛选 全细胞生物催化 两相反应 

分 类 号：TQ033[化学工程]