α-亚麻酸对草酸钙结石大鼠细胞因子的影响  

Experimental study of α-Linolenic acid's effect on cytokines in rats with calcium oxalate urolithiasis

作　　者：王军[1] 叶章群[2] 宋波[1] 金锡御[2] 杨为民[1] 王少刚[1] 周四维[2] 

机构地区：[1]第三军医大学西南医院全军泌尿外科中心,重庆400038 [2]华中科技大学同济医院泌尿外科

出　　处：《临床泌尿外科杂志》2005年第10期634-636,共3页Journal of Clinical Urology

摘　　要：目的:探讨炎性细胞因子IL-1α、L-1β、IL-6和TNF-α在尿结石形成中的作用,以及α-亚麻酸(α-LNA)对其的抑制影响。方法:将45只雄性Wistar成年大鼠随机分成3组,分别接受普通饲料加自来水饮水(A组)、普通饲料加诱石剂饮水(B组)和普通饲料加诱石剂饮水并每天用苏子油灌胃(2 g.只-1.d-1)(C组)。于喂养第8周后检测各组大鼠血细胞因子水平。利用体外培养的大鼠肾系膜细胞(RMC),设立空白对照(a组)、IL-6处理(b组)、EPA10μmol加IL-6处理(c组)、EPA50μmol加IL-6处理(d组)及无水乙醇处理(e组),用放免法检测培养上清中PGE2含量。结果:C组和A组IL-1αI、L-6水平显著低于B组,三组间IL-1β、TNF-α水平差异无统计学意义。细胞培养上清PGE2含量,a、c、d组显著低于b组,d组显著低于c组,a、e两组间差异亦无统计学意义。结论:炎性细胞因子IL-1αI、L-6可能参与了尿石形成过程,α-亚麻酸可能通过抑制炎性细胞炎子的产生而对尿石形成起保护作用。Objective：To investigate the possible role of cytokines interleukin （IL）-1β, IL-1α, IL-6 and TNFα in urinary stone formation and the inhibiting effect of α-Linolenic acid on these cytokines. Methods： Experiment 1 ： 45 male adult wistar rats were divided into 3 groups： Group A was negative control, Group B was stone group （drinking 1% of ethylene glycol plus 1% of muriat water）, Group C was perilla frutescens Britt · oil group （drinking 1% of ethylene glycol plus 1% of muriat water, meanwhile, tube feeding with perilla frutescens Britt · oil （10 g · kg^-1·d^-1）. After 8 weeks, serum IL^-1β, IL-1α, IL-6 and TNFa were determined. Experiment 2： In vitro cultured RMCs were divided into 5 groups： Group a： control; Group b： pretreated with IL6; Group c： 10 μm EPA plus IL6; Group d： 50 μm EPA plus IL6 for 24 hours; Group e： pretreated with absolute alcohol. The PGE2 concentration in supernatants of RMCs was measured by radioimmunoassay. Results： Serum concentration of IL-1α, IL6 in group A and C rats were significantly lower comparing with those in B group rats. There weren＇t significantly differences among three groups for IL-1β and TNFα concentration. The PGE2 concentration in group a, c, d were significantly lower than that of group b. Group d was significantly lower than that of group c. There weren＇t difference between group a and e. Conclusions： Cytokines IL-1α and IL-6 may participate in urinary tone formation, αLinolenic acid may prevent urinary stone formation by the inhibitory role on these cytokines.

关 键 词：尿石症 细胞因子 Α-亚麻酸 

分 类 号：R691.4[医药卫生—泌尿科学]