富血小板血浆对牙周膜成纤维细胞损伤模型影响的研究  

Effect of Platelet-rich plasma(PRP) on Periodontal Ligament Fibroblasts in an in vitro Wound Healing Model.

作　　者：钟声[1] 闫福华[1] 卢友光[1] 林敏魁[1] 赵欣[1] 

机构地区：[1]福建医科大学口腔医学院牙体牙髓科,福建福州350002

出　　处：《口腔医学研究》2005年第5期504-506,共3页Journal of Oral Science Research

基　　金：福建省科技厅重大课题资助项目(编号:2001Z021);福建省卫生厅青年基金资助项目(编号:2004-1-24)

摘　　要：目的:探讨应用富血小板血浆(PRP)对狗牙周膜成纤维细胞(PDLFs)损伤模型的愈合有无影响。方法:采用组织块法培养狗牙周膜成纤维细胞,取第四代细胞接种于12孔板,待细胞融合后制作直径约8 mm的损伤模型。用含1%PRP和10%PRP的培养液继续培养,于损伤后第1、4、7、9、11天分别检测各组细胞数量及细胞覆盖损伤区的情况。结果:1%PRP和10%PRP均可促进损伤PDLFs的移行和增殖。结论:1%PRP和10%PRP均可促进牙周膜成纤维细胞损伤模型的愈合。Objective： The aim of this study was to evaluate the influence of platelet - rich plasma （PRP） on cultured periodontal ligament fibroblasts, using an in vitro wound healing model. Methods： Platelet -rich plasma （PRP） was isolated by two - step centrifugation. Primary PDLFs were obtained from mongrel normal first premolar. The 4th passage of PDLFs were cultured in 12 - well chamber plates. The cells were grown to confluence and in vitro wounds were mechanically created, by removing a 8 rnm diameter cells within a circular area using a sterilized silicon bung. The wells were then incubated for 1, 4, 7, 9 and 11 days and the healing speed of these groups was evaluated. Results ： The proliferation and the migration of cells were enhanced by addition of 1% PRP and 10% PRP. Conclusion： PRP appears to exert an influence on cells that is compatible with improved wound healing.

关 键 词：富血小板血浆 牙周膜成纤维细胞 生长因子 损伤愈合模型 

分 类 号：R781.4[医药卫生—口腔医学]