尼古丁对人牙周韧带成纤维细胞增殖和细胞周期的影响  

The Effects of Nicotine on the Proliferation and Cell Cycle of PDLFs.

作　　者：高丽[1] 刘琪[1] 冯萍[1] 葛颂[1] 

机构地区：[1]遵义医学院附属口腔医院口腔内科,贵州遵义563003

出　　处：《口腔医学研究》2005年第5期507-509,共3页Journal of Oral Science Research

基　　金：省卫生厅优秀青年基金资助项目(编号:D-164);省教育厅基金资助项目(编号:C-200)

摘　　要：目的:研究尼古丁(n icotine)对人牙周韧带成纤维细胞(periodontal ligam ent fibrob lasts,PDLFs)增殖和细胞周期的影响。方法:用不同浓度尼古丁分别在不同时间作用于PDLFs,用MTT比色法及流式细胞仪(flow cytom e-try,FCM)检测细胞增殖和细胞周期。结果:尼古丁能明显抑制PDLFs增殖,呈剂量依赖性和时间依赖性;FCM结果表明,经尼古丁作用后,PDLFsG0-G1期细胞百分比显著增加(P<0.01),G2-M期细胞、S期细胞百分比显著下降(P<0.01),反映细胞增殖和性的增殖指数PI值(S+G2M)随着浓度的递增而下降。结论:尼古丁可抑制PDLFs增殖并减少细胞DNA合成。Objective： The aim of this study was to investigate the effects of nicotine on periodontal ligament fibroblasts （PDLFs）, Methods： PDLFs were cultured in the presence of nicotine at various concentrations and periods; MTT colorimetric assay and flow cytometry （ FCM ） were used to test the cell proliferation and cell cycle. Results ： Nicotine could significantly inhibit PDLFs proliferation in a dose - dependent manner and a time - dependent manner. The results of FCM analysis showed that S and G2 - M of cell cycle decreased, while Go - G1 increased, when the cells were stimulated by nicotine, Conclusion： Nicotine may inhibit cell proliferation and DNA synthesis of PDLFs.

关 键 词：尼古丁 牙周韧带成纤维细胞 细胞增殖 细胞周期 

分 类 号：R781.4[医药卫生—口腔医学]