3,5-二硝基水杨酸(DNS)比色法测定马铃薯还原糖含量的研究  

Applying 3,5-dinitrosalicylic Acid(DNS) Method to Analyzing the Content of Potato Reducing Sugar

作　　者：朱海霞[1] 石瑛[1] 张庆娜[1] 陈伊里[1] 

机构地区：[1]东北农业大学,黑龙江哈尔滨150030

出　　处：《中国马铃薯》2005年第5期266-269,共4页Chinese Potato Journal

基　　金：国家"863"计划项目(2004AA241133)资助

摘　　要：试验针对3,5-二硝基水杨酸(DNS)比色法测定马铃薯还原糖含量的吸收光谱及其对测定值可靠性的影响因素进行了研究。找到了分析马铃薯还原糖的最佳条件:λ射波长为510nm,显色剂用量为6mL,在沸水浴的显色时间范围为12～15min,结果表明该方法的灵敏度得到了提高。由于该方法具有方便、安全、成本低等优点,适合于常规实验室马铃薯还原糖批量和定量的测定。3,5-dinitrosalicylic acid （DNS） method was used to analyze the content of reducing sugar by using spectrophotometry, and the wave-length and some relevant factors were researched in analyzing the content of potato reducing sugar with DNS method. The best conditions for analyzing the content of potato reducing sugar had been established. These were： wave-length： 510 nm; DNS solution： 6 mL; and duration for color development in water bath： 12-15 min. The sensitivity of the method had been improved. The experiment had confirmed that the improved DNS method was simple, safe and easy to conduct. The method can be used in common laboratories for quantitative analysis.

关 键 词：马铃薯 还原糖 比色法 3 5-二硝基水杨酸 

分 类 号：S532[农业科学—作物学]