紫球藻胞外多糖的分离纯化与性质分析  

Seperation,Purification and Property of Extracellular Polysaccharide from Porphyridium sp.

作　　者：朱桂芳[1] 魏东[1] 刘石生[1] 郭祀远[1] 陈峰[1] 

机构地区：[1]华南理工大学轻工与食品学院,广州510640

出　　处：《天然产物研究与开发》2005年第5期542-548,共7页Natural Product Research and Development

基　　金：广东省自然科学基金项目(5006524);广东省科技攻关项目(2005B20301017)

摘　　要：采用Q Sepharose Fast Flow凝胶,从紫球藻胞外多糖(ESPS)获得3个组分ESPS0,ESPS1.0和ESPS1.6,它们经过Sephadex G200凝胶过滤纯化后,紫外光谱、红外光谱、氨基酸分析、单糖组成分析表明,ESPS0中未测出蛋白,ESPS1.0和ESPS1.6的蛋白含量分别为7.8%和7.6%,三者硫酸基含量分别为16.3%,11.6%和8.3%,同时分别在201 nm,207 nm和199 nm处出现特征吸收峰,而在280 nm和260 nm波长处无吸收峰。ESPS0、ESPS1.0中单糖的连接方式为β-糖苷键,而ESPS1.6中主要为α-糖苷键。ESPS1.0、ESPS1.6都含有十七种氨基酸。三种组分都是由D-木糖、D-葡萄糖、D-甘露糖、D-艾杜糖以不同的比例所组成。By using Q Sepharese Fast Flow gel, extracellular polysaccharide ESPS from Porphyridium sp. were seperated into three fractions,ESPS0,ESPS1.0 and ESPS1.6. They were purified further by Sephadex G-200 column chromatography. And UV spectrum, FF-IR, amino acid analysis and monosaccharide composition analysis were carried out. The results showed that protein content of ESPS0, ESPS1.0 and ESPS1.6 were 0,7.8 % and 7.6 %, and SO3- content were 16.3 %, 11.6 % and 8.3 %, respectively. Meanwhile,it is shown that the specific UV absorption was at 207 ran,201 nm and 199 nm,no absorption at 280 nm and 260 nm β-linkage of monosaccharides in ESPS0,ESPS1.0 and α-linkage in ESPS1.6 were determined. ESPS1.0 and ESPSI.6 ale consist of 17 types of amino acids. These fractions were made from D-xylose, D-glucose, D-mannose, D-idose in different proportions.

关 键 词：紫球藻 胞外多糖 离子交换层析 性质 

分 类 号：O629.12[理学—有机化学] Q946.91[理学—化学]