高效液相色谱法分析测定越桔果渣中的黄酮甙元  

Qualitative and Quantitative Analysis of Flavonoid Aglycones from Fruit Residue of Vaccinium vitis-idaea L.by HPLC

作　　者：潘一峰[1] 瞿伟菁[1] 李家贵[1] 顾于蓓[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《天然产物研究与开发》2005年第5期642-644,641,共4页Natural Product Research and Development

基　　金：上海市科技发展基金项目(024358013)

摘　　要：采用高效液相色谱法对越桔果渣中黄酮甙元进行定性和定量分析。用甲醇和水(60:40)以0.8 mL/min流速进行洗脱,368 nm波长检测越桔果渣水解液中黄酮类成分并测定其含量。结果:越桔果渣中黄酮甙元主要有杨梅素和槲皮素,其含量分别为:0.0206%和0.0686%。该方法测定样品简单易行,分离效果佳,灵敏度高,重现性好,结果准确可靠。This paper is to do qualitative and quantitative analysis of the flavonoid aglycones in the fruit residue of Vaccinium vitis-idaea L.by HPLC.Two types of flavonoid aglycones,myricetin（0.0206% ）and quercetin（0.0686% ）,were detected in the fruit residue of Vaccinium vitis-idaea L.by KPLC using methanol and water （ 60 ： 40 ） as mobile phase at flow rate of 0.8 mL/min. The peak was detected at 368 nm. The method is simple,effective,sensitive and feasible.

关 键 词：越桔 高效液相色谱 黄酮甙元 

分 类 号：R284.1[医药卫生—中药学] Q946.91[医药卫生—中医学]