达吉胶囊中纤维素酶的活力测定  

Assay for the cellulase activity of Dages capsule

作　　者：佟爱东[1] 黎宇君[2] 

机构地区：[1]广州市药品检验所,广东广州510160 [2]广东药学院2003届药学系,广东广州510240

出　　处：《中国生化药物杂志》2005年第5期299-301,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的测定达吉胶囊中纤维素酶的活力。方法以羧甲基纤维素钠为底物与酶溶液在40℃反应30 min后,用3,5-二硝基水杨酸显色法或索默季砷钼酸盐显色法测定其葡萄糖的生成量,计算酶活力。结果用3,5-二硝基水杨酸显色法与索默季砷钼酸盐显色法测定3批供试品纤维素酶活力分别为标示量的165%,108%,150%与161%,110%,148%。结论2种方法测定结果相同。3,5-二硝基水杨酸显色法测定达吉胶囊中纤维素酶的活力方法简便,精密度较好,结果准确,适用于产品的质量控制。Purpose To determine the cellulase activity of Dages capsule. Methods 0. 39% carboxymethylcellulose sodium pH 4.5 in acetate buffer solution （0.1 mol/L） 4 ml is reacted with sample solution （approx. 1.6 Unit/ml） 1 ml at 40℃ for 30 minutes. Using DNS reagent or Somogyi-Nelson reagent to measure the glucose quantity produced, then calculating the activity of the cellulase. Results Two methods＇ results of the three samples are 165 %, 108 %, 150 % and 161%, 110%, 148% respectiveh. Conclusion The results of two methods are the same. The DNS method is simple, accurate and suitable for quality control.

关 键 词：达吉胶囊 纤维素酶 葡萄糖测定 

分 类 号：R977.3[医药卫生—药品] R927.2[医药卫生—药学]