用二次谐波成像技术研究经飞秒激光切削后角膜变化  

Second Harmonic Generation Microscopy Imaging of Cornea after Femtosecond Laser Intrastromal Ablation

作　　者：王艳艳[1] 韩蒙 孙辉 JOSEF Bille 任秋实[1] 

机构地区：[1]上海交通大学激光与光子生物医学研究所 [2]Heidelberg大学Kirchhoff,Institute,for,Physic,Im,Neuenheimer,Feld227,D-69120,Heidelberg,Germany

出　　处：《激光生物学报》2005年第5期321-326,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(60371021);上海市光科技专项基金项目(036105015)

摘　　要：本文用二次谐波成像技术(second harmonic generation SHG)来研究飞秒激光切削后角膜结构的变化。在生物学研究,材料科学等方面都有很广泛应用的SHG成像技术能在不破坏的角膜情况下获得高对比度的角膜层析图像,分辨率为500 nm,实验装置是利用现有的双光子显微镜。本文还根据成像结果评价了飞秒激光在角膜切削中的质量,为飞秒激光微米级的精确切削和临床应用提供了实验支持。In this article Second Harmonic Generation （SHG） microscopy is utilized to investigate the structrual modification of cornea after laser treatment. With SHG microscopy which is widely used in biological researches, material sciences, a three-dimensional image stack of eomea can be abtained by optical sectioning noninvasively, the resolution is 500 nm. The imaging setup based on two photon microscope. From the imaging results, the peformance of femtosecond laser in comca ablation is evaluated, femtosecond laser can really ablate comea precisely within several um range, the conclusion of this experiment can support femtosecond laser to be applicated in clinics.

关 键 词：光学 飞秒激光 二次谐波成像 多光子显微镜 角膜 

分 类 号：Q631[生物学—生物物理学] R778[医药卫生—眼科]