赤子爱胜蚓5个Dmrt基因的序列分析  

Sequence Analysis of Five Dmrt Genes in Eisenia foetida

作　　者：彭巧玲[1] 程子华[1] 蒲友光[1] 葛亚东[1] 聂刘旺[1] 

机构地区：[1]安徽师范大学生命科学学院安徽重要生物资源保护与利用研究重点实验室,中国安徽芜湖241000

出　　处：《激光生物学报》2005年第5期343-347,共5页Acta Laser Biology Sinica

基　　金：安徽省自然科学基金(01043202);重要生物资源保护与利用安徽省重点实验室;生物环境与生态安全安徽省高校重点实验室;安徽省教育厅自然科学基金重点项目(2001kj113ZD)

摘　　要：本文采用简并PCR技术,扩增了赤子爱胜蚓Dmrt基因的DM结构域,经序列分析,获得了Dmrt基因家族的5个成员EfDmrt2、EfDmrt3、EfDmrt4a、EfDmrt4b、EfDmrt4c。与其他动物相关的Dmrt基因进行聚类分析,结果表明,不同进化地位动物的Dmrt基因DM域编码序列存在高度的同源性,显示Dmrt基因在系统进化上高度保守,序列上的相似性可能暗示它们在功能上的保守性。In this paper, degenerate PCR was used to amplify the conserved DM domains of Dmrt genes in Eisenia foeticla. The PCR products were approximately 150 bp. After DNA cloning and sequencing analysis, five different DM sequences were identified and named as EfDmrt2, EfDmrt3, EfDmrt4a, EfDmrt4b and EfDmrt4c, according to its name Eisenia foetida. The amino acid sequences encoded by Dmrt genes from different species showed a high degree of sequence homology, as revealed in phylogenic tree constructed. The results further indicated that Dmrt genes were highly conservative in phylogeny and the strong evolutionary conservation of this gene family may be of importance in developmental process.

关 键 词：赤子爱胜蚓 DMRT基因 简并PCR 

分 类 号：Q78[生物学—分子生物学]