黄连解毒汤高效液相色谱分析方法的建立  

Establishment of Method for Analysis of Decoction Huanglianjiedutang by HPLC

作　　者：任玲玲[1] 薛兴亚[1] 徐青[1] 梁鑫淼[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023

出　　处：《世界科学技术-中医药现代化》2005年第5期41-44,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金重点项目(NO.20235020):中药药效组分指纹图谱分析方法的研究;中国科学院知识创新工程重要方向项目(NO.KGCX2-SW-213):基于现代理论和技术的复方中药系统研究

摘　　要：黄连解毒汤由黄连、黄芩、黄柏、栀子四味中药组成,主要包含生物碱类(黄连、黄柏),黄酮类(黄芩)和环烯醚萜类(栀子)三大类成分,其理化性质差别较大,在分别进行高效液相色谱(HPLC)分析时适用的流动相差别较大。本文比较了不同浓度的甲酸-三乙胺流动相添加剂对黄连解毒汤水提取物高效液相色谱(HPLC)分析的影响,提出了一种稳定、重复的HPLC分析方法:柱子为HypersilODS2色谱柱(250×4.6mmi.d,5μm),流动相组成为0.3%(V/V)甲酸-0.3%(V/V)三乙胺的水溶液与0.3%(V/V)甲酸-0.3%(V/V)三乙胺的乙腈溶液,检测波长260nm。这种方法能够同时分析复方中的生物碱类、黄酮类和环烯醚萜类物质,为类似的成分复杂中药提取物的分析提供了参考。The decoction Huanglianjiedutang consists of four medicinal plants, i.e. Coptis japonica Makino, Scutellaria baricaleins Georgi, Phellodendron amuranse Ruprecht and Gardenia jasminonoides Ellis and its constituents mainly include three groups of flavonoids, alkaloid and iridoid glycosides with great difference of chemico-physical properties, which assume different mobile phases when analyzed by HPLC respectively. This article describes via comparison the influence of formi acid-triethylamine mobile phase additive with different concentrations on the analysis of the aqueous extract of the decoction Huanglianjiedutang by HPLC, thereby having developed a stable and repetitive method of HPLC analysis, in which the analysis is composed of a chromatographic column Hypersil ODS2(250 × 4.6mm i.d, 5 um particle size), a mobile phase consisting of the aqueous solution of 0.3%(v/v) formic acid-0.3%(v/v) triethylamine and the acetonitrile solution of 0.3%(v/v) formic acid-0.3(v/v) triethylamine, and an ultraviolet detector with the wavelength 260nm. This method can be used to analyze alkaloids, flavonoids and iridoid glycosides of TCM compound drugs at the same time, thas providing a refernce for the analysis of TCM extracts with complex constituents.

关 键 词：黄连解毒汤 HPLC 甲酸 三乙胺 高效液相色谱(HPLC) 黄连解毒汤 色谱分析方法 流动相添加剂 环烯醚萜类 HPLC分析 生物碱类 中药提取物 中药组成 

分 类 号：R155.5[医药卫生—营养与食品卫生学] R285.5[医药卫生—公共卫生与预防医学]