必需磷脂防治酒精性脂肪肝的实验研究  

Prevention and treatment on alcoholic fatty liver by Essentiale

作　　者：冯志强[1] 沈志祥[2] 谭诗云[2] 漆楚波[3] 

机构地区：[1]温州医学院附属台州医院老年医学院 [2]武汉大学人民医院消化内科,临海317000 [3]湖北省肿瘤医院病理科

出　　处：《胃肠病学和肝病学杂志》2005年第5期480-482,486,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨必需磷脂(易善复)对酒精性脂肪肝(Alcoholic fatty liver;AFL)的作用及其可能的机制,为其临床防治提供一种有效的方法。方法Wistar雄性大鼠36只,随机分为4组。1组为脂肪肝模型组,2～3组为治疗组,4组为空白对照组。1～3组大鼠均按15ml/kg用52%,v/v二锅头白酒灌胃,喂以特制饲料。所有大鼠均以腹腔注射方式给药,1组用5ml/kg生理盐水腹腔注射,2、3组各按10mg/kg、30mg/kg给予易善复;4组以15ml/kg生理盐水灌胃,喂以普通饲料。第4周末所有大鼠称重,处死动物,取全肝,检测血浆、肝匀浆SOD、MDA、GSHPx;肝匀浆ATPase;取肝脏进行病理组织学检查。结果病理组织学所见:1组:4周后出现典型脂肪肝病变。2组:表现为轻度脂肪肝,少数炎性细胞浸润;3组:轻微脂肪沉积;4组:肝小叶及肝细胞结构正常。生化指标:1组与2～4组相比,血清、肝匀浆SOD、MDA、GSHPx、肝匀浆ATPase活性均有显著性差异(P<0.05);2组与3组间,上述指标亦有差异(P<0.05)。结论易善复能抑制AFL的形成,且成量效关系。Objective To study the prevention effect of Essentiale on alcohclic fatty liver in rats. Methods Sixty- six wistar male rats were randomly divided into four groups： model group（group 1, intragastric infusion of spirits by the dose of 15 ml/kg, fed with high fat and iron diet）, treatment groups（group 2 - 3, intragastric infusion of spirits by the dose of 15 ml/ kg, fed with high fat and iron diet）, Control group（group 4, intragastric infusion of saline by the dose of 15 ml/kg, fed with ordinary diet）. Among the treatment groups： group 2 were injected with Essentiale（ 10 mg/kg）,while group 3 were treated with Essentiale according to 30 mg/kg All rats were administrated once a day. And by the end of the 4th week, all rats were weighted, and sacrificed. SOD, MDA,and GSH-Px of the plasm, and SOD, MDA, GSH-Px, and ATPase of the liver homogenate were measured respectively. Results In the model group： the classic AFL lesions were seen in the hepatocyte. While slight fatty liver as observed in the 2nd group. Slight fatty deposition was only observed in the 3rd group. The structure of the hepatocytes was normal in the 4th group. Compared with the 1st group, SOD, MDA, GSH-Px of the plasm and SOD, MDA, GSH-Px, ATPase of the liver Homogenate in group 2 to 4 were statistically different（ P 〈 0.05） .The differences of the above index between 2nd, 3rd and 4th were significant（ P 〈 0.05）. Conclusion Essentiale can prevent the formation of the AFL. And the effect was dose-dependent.

关 键 词：酒精 脂肪肝 易善复 

分 类 号：R575.5[医药卫生—消化系统]