β-APRIA检测中的一些影响因素探讨  

Study on the Factors Affecting β-AP RIA Results

作　　者：余斌伟[1] 徐尚敖[1] 陆志峰[1] 徐林燕[1] 

机构地区：[1]浙江省江山市人民医院,324100

出　　处：《放射免疫学杂志》2005年第5期354-356,共3页Journal of Radioimmanology

摘　　要：目的:探讨血清标本的保存、标本溶血、脂浊血清、细菌污染等对β-AP检测结果的影响。方法:取9例血清标本置4℃保存,观察β-AP含量与保存期的关系;人为造成标本溶血观察β-AP含量;脂浊血清与脱脂血清β-AP含量比较;细菌污染血清标本后观察β-AP含量变化。结果:血清标本4℃贮存4dβ-AP结果无显著变化(P>0.05);溶血标本β-AP值明显增高(P<0.01);脂浊血清β-AP含量较脱脂血清高(P<0.05);细菌污染标本后对β-AP含量检测有影响。结论:β-AP在4℃保存4d含量稳定,在室温中迅速降解。标本溶血、脂浊、细菌污染均可致β-AP检测结果造成异常变化,在实际工作中必须予以重视。Objective To study the effect of ① storage time ② hemolysis ③ excessive lipid content ④ bacterial contamination on the tested serum β - AP RIA results. Methods ① Specimen was stored at 4℃ and β- AP contents determined daily for seven consecutive days （9 specimens）. ② specimen was assayed, then the clot forcibly broken to cause hemolysis and the serum re - assayed （8 specimens）. ③ Hyperlipemic specimen was assayed, the excess lipid extracted with ether and serum re -assayed （8 specimens）. ④ Specimen was assayed and left at room temperature for a few days. The specimen was re - assayed for several times after bacterial growth （10 specimens）. Results serum β- AP contents were stable up to 4 days under 4℃ （P 〉 0.05）. Serum β -AP contents increased significantly after hemolysis （P 〈0.01 ）. β - AP contents in hyperlipemic samples decreased significantly after ether treatment （P 〈0.01 ）. With bacterial growth, the β- AP contents could increase or decrease, depending upon the type of bacteria. Conclusion Serum β - AP was stable up to 4 days at 4℃, but rapid degraded under room temperature. Moreover, hemolysis, hyperlipemia and contamination would all affect the RIA result and should be carefully dealt with in daily work.

关 键 词：血清保存 溶血 脂浊 细菌污染 β-类淀粉样蛋白含量 放射免疫分析 血清β-AP RIA检测 血清标本 含量比较 细菌污染 标本溶血 4℃保存 检测结果 含量变化 

分 类 号：R749.160.2[医药卫生—神经病学与精神病学] R714.22[医药卫生—临床医学]