CpG ODN增强rHBsAg免疫小鼠抗体产生及淋巴细胞增殖效应  

Effect of CpG ODN on Anti-HBs and Lymphocyte Proliferation of Splenocytes in Mice Immunized with rHBsAg

作　　者：石艳春[1] 郑源强[2] 梁浩[3] 杨贵贞[3] 

机构地区：[1]内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室,呼和浩特010021 [2]华北煤炭医学院生物科学系,河北唐山063000 [3]吉林大学医学部基础医学院免疫学教研室,长春130021

出　　处：《科技导报》2005年第11期27-29,共3页Science & Technology Review

基　　金：内蒙古自然科学基金项目(200508010905)

摘　　要：目的:探讨合成含CpG基序的寡核苷酸(CpGODN)对重组乙型肝炎表面抗原(rHBsAg)增强小鼠特异性抗体产生及淋巴细胞增殖反应的效应。方法:采用Balb/c小鼠作为免疫实验动物,经后腿胫骨前肌免疫2次,ELISA法检测血清乙型肝炎表面抗体(抗-HBs)效价;3H-TdR掺入法测定淋巴细胞增殖反应。结果:加CpGODN组的抗-HBs效价较rHBsAg组明显增高,且维持时间长;淋巴细胞增殖反应显著增强。结论:CpGODN对小鼠抗-HBs产生具有明显的增强作用,且可以有效刺激小鼠淋巴细胞的增殖反应。To investigate effects of synthetic oligodeoxynuclentide containing CpG motif （CpG ODN） on specific antibody production and lymphocyte proliferation to recombinant hepatitis B surface antigen （rHBsAg）. Methods： Balb/c mice received into back leg tibialis anterior （TA） muscle for two times ; Detection of anti-HBs titer in serum by ELISA;The lymphocyte proliferations are tested by ^3H-TdR incorporation. Results： The anti-HBs titer of plus CpG ODN group is higher than that of rHBsAg group obviously, and the antibody persistent time is also longer. Nevertheless, the proliferation of splenocytes of the group plus CpG ODN is much stronger. Conclusion： CpG ODN has enhanced efficacy for anti-HBs production and stimulated the splenocytes proliferation in mice.

关 键 词：疫苗 CPG ODN 乙型肝炎表面抗原 佐剂 

分 类 号：R392.1[医药卫生—免疫学]