三倍体草鱼染色体限制性内切酶带分析  

CHROMOSOMAL RESTRICTION ENDONUCLEASE DIGESTION OF A TRIPLOID GRASS CYPRINID(Ctenopharyngodon idellus)

作　　者：任修海[1] 

机构地区：[1]上海大学生命科学院

出　　处：《Zoological Research》1996年第2期187-192,共6页动物学研究（英文）

基　　金：国家自然科学基金

摘　　要：本文报道运用Ｇｉｅｍｓａ染色、Ｃ－带显带、ＮＯＲ－显带和多种限制性内切酶消化对草鱼三倍体的染色体进行分析。其中，内切酶ＨａｅⅢ和ＨｉｎｄⅢ等使染色体产生Ｇ－带，其余内切酶产生与常规Ｃ－带不同的分化，即所谓修饰Ｃ－带。实验结果表明，限制性内切酶可使鱼类染色体产生多种带型，各种显带技术的结合使用，可探讨鱼类染色体及染色质结构与进化。In this paper,we report on the cytogenetic detection of a triploid of Ctenophryngodon idellus by restriction endonuclease digestion and conventional banding techniques.Restriction enzymes Hae Ⅲ,Alu Ⅰ(recognizing four base sequences respectively) and Hind Ⅲ(recognizing a six base sequence) induced linear differentiations (G-bands).The isoschizomers HPall and MapⅠ which recognize 4 base sequences resulted in modified C-bands differing from those produced by conventional C-banding.These findings clearly show that restriction endonucleases are capable of producing G banding patterns in fish chromosomes and that restriction enzyme digestion can be very useful in the analysis of fish chromatin orgallizatin.

关 键 词：三倍体 草鱼 鱼纲 染色体显带 限制性内切酶 

分 类 号：Q959.468[生物学—动物学]