AG 1478对GRC-1细胞的诱导凋亡作用及其机制  

Effect of epidermal growth factor receptor inhibitor tyrophostin AG1478 on hu man renal cell carcinoma line of GRC-1

作　　者：张顺[1] 刘凡[1] 张运涛[1] 汪涌[1] 

机构地区：[1]第四军医大学唐都医院泌尿外科,陕西省西安市710038

出　　处：《实用医学杂志》2005年第20期2227-2229,共3页The Journal of Practical Medicine

摘　　要：目的:探讨表皮生长因子受体阻滞剂TyrophostinAG1478对肾癌GRC-1细胞的诱导凋亡作用及其机制,为肾癌的生物学特征研究和药物治疗提供理论依据。方法:将不同浓度的AG1478作用于体外培养的肾癌GRC-1细胞,以研究表皮生长因子受体信号转导阻滞剂对细胞增殖和细胞周期分布的影响。结果:AG1478抑制肾癌GRC-1细胞的增殖,诱导细胞周期停留在G1期,诱导肾癌GRC-1细胞凋亡。结论:表皮生长因子受体特异性阻滞剂可抑制肾肿瘤细胞的增殖,有望成为抗肾脏恶性肿瘤药物。Objective To investigate the biological effect of tyrophostin AG1478 on human renal cell carcinoma line GRC-1 in vitro and to explore the possibility of high selective inhibitor of epidermen growth factor receptor （EGFR） as a kind of anti-cancer drug, Methods Mter the human renal cell carcinoma llne GRC-1 was exposed in vitro to desired concentrations of tyrophostin AG1478, the biological behaviors were observed by MTT assay and flow cytometer. Results Tyrophostin AG1478 could not only inhibit the proliferation of GRC-1 cells and induce cell cycle rest on G; phase, but also induce apoptosis of GRC-1 cells. Conclusion High selective inhibitor of EGFR can inhibit the proliferation and promote the apoptosis of GRC-1 cells.

关 键 词：肾肿瘤 受体 表皮生长因子 细胞凋亡 Tyrophostin AG1478 GRC-1细胞 诱导凋亡作用 表皮生长因子受体 细胞周期分布 信号转导阻滞剂 

分 类 号：R737.11[医药卫生—肿瘤] R282.71[医药卫生—临床医学]