环氧合酶-2在卵巢癌组织中的表达  

Expression of cyclooxygenase-2 in ovarian cancer

作　　者：李晓艳[1] 王泽华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉市430022

出　　处：《实用医学杂志》2005年第20期2254-2255,共2页The Journal of Practical Medicine

摘　　要：目的:探讨环氧合酶-2(cyclooxygenase-2,COX-2)在卵巢癌组织中的表达及意义。方法:应用免疫组化和RT-PCR技术检测正常卵巢和卵巢癌组织中COX-2的表达情况。结果:检测的38例卵巢癌组织COX-2蛋白表达阳性23例,阳性表达率61%,33例表达COX-2mRNA,阳性表达率为87%;20例正常卵巢组织COX-2蛋白与COX-2mRNA均呈阴性表达。结论:COX-2在卵巢癌组织高表达,并在其发生发展中起重要作用。Objective To investigate the expression of cyclooxygenase-2（COX-2）in ovarian cancer. Methods RT-PCR and immunohistochemlcal method were used to investigate the expression of cyclooxygenase-2 in 38 patients with ovarian cancer. Results COX-2 protein expressions were detected in 23 of 38 ovarian cancers and the positive rate was 61%. COX-2 mRNA expressions were detected in 33 of 38 ovarian cancers and the positive rate was 87%. No COX-2 protein and mRNA expression was detected in 20 normal ovaries. Conclusion Increased expression of COX-2 protein and mRNA in human ovarian cancer, and it might be the imoortant factor in develooment of ovarian cancer.

关 键 词：卵巢肿瘤 环氧化酶-2 免疫组织化学 逆转录聚合酶链反应 卵巢癌组织 环氧合酶-2 COX-2MRNA PCR技术检测 阳性表达率 COX-2蛋白 

分 类 号：R737.31[医药卫生—肿瘤] R735.2[医药卫生—临床医学]