软枣猕猴桃试管苗叶片和茎段的愈伤组织诱导及植株再生  

CALLUS INDUCTION AND PLANT REGENERATION FROM LEAVES AND STEM SEGMENTS OF IN VITRO SHOOTS OF ACTINIDIA ARGUTA

作　　者：张远记[1] 钱迎倩[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《西北植物学报》1996年第2期137-141,共5页Acta Botanica Boreali-Occidentalia Sinica

摘　　要：从软枣猕猴桃（Ａｃｔｉｎｉｄｉａａｒｇｕｔａ（Ｓｉｅｂ．ｅｔＺｕｃｃ．）Ｐｌａｎｃｈ．ｅｘＭｉｑ．）试管苗茎段和叶片诱导出愈伤组织并得到再生植株。茎段外植体容易愈伤化，但其愈伤组织难以分化；叶片外植体不易愈伤化，但其愈伤组织容易分化。ＭＳ培养基分别附加ＢＡＰ（０．５，１．０和２．０ｍ／Ｌ）、Ｋｉｎ（０．５，１．０和２．０ｍｇ／Ｌ）、ＴＤＺ（０．０００１，０．０１和１．０ｍｇ／Ｌ）或ＣＰＰＵ（０．０００２５、０．０２５和２．５ｍｇ／Ｌ，配合ＩＡＡ０．１ｍｇ／Ｌ）都不能诱导芽的分化，而ＭＳ附加玉米素（０．５，１．０，２．０和３．０ｍｇ／Ｌ）能有效地诱导芽分化．其中以２．０ｍｇ／Ｌ玉米素效果最好。Calli were induced and whole plants were regenerated from in vitro culture of leaf and stem segments of Actinidia arguta (Sieb. et Zucc. )Planch. ex Miq.. Leaf explants were difficult to induce callus but the calli were easy to differentiate buds;on the contrary,stem segments were easy to callusise but hard to obtain bud regeneration. Bud regeneration was not obtained on MS medium supplemented with BAP (0. 5, 1. 0 and 2.0mg/L),Kin (0. 5, 1. 0 and 2. 0mg/L),TDZ (0. 0001, 0. 01 and 1. 0mg/L),and CPPU (0. 00025, 0. 025 and 2. 5mg/L, in the presence of 0. 1mg/L IAA), respectively. In contrast,MS medium added with zeatin (0. 5, 1. 0, 2. 0 and 3. 0mg/L) was found to be proper for bud differentiation, and the best result was obtained from MS medium with 2. 0mg/L zeatin.

关 键 词：猕猴桃 软枣猕猴桃 组织培养 植株再生 

分 类 号：S663.903.5[农业科学—果树学]