灯盏花素对缺血再灌注沙土鼠海马ATP含量变化和兴奋性氨基酸释放的影响  

Effects of erigeron on ATP content and release of excitatory amino-acids of the hippocampus after cerebral ischemia/reperfusion injury in gerbils

作　　者：张焰[1] 陈群[2] 顾卫东[1] 丁浩中[1] 曾因明[2] 

机构地区：[1]无锡市第四人民医院麻醉科,214062 [2]徐州医学院麻醉学系

出　　处：《江苏医药》2005年第11期843-845,F0002,共4页Jiangsu Medical Journal

摘　　要：目的研究灯盏花素对缺血再灌注沙土鼠海马ATP含量、ATP酶活性和兴奋性氨基酸释放的影响。方法脑缺血10 min建立沙土鼠缺血再灌注模型。90只沙土鼠分为假手术组、脑缺血组和灯盏花素组。测定脑缺血后和再灌注1、12和24 h海马ATP含量、ATP酶活性和兴奋性氨基酸含量。结果脑缺血组,ATP、Na+-K+-ATP酶和Ca2+-ATP酶含量明显下降(P<0.01),谷氨酸(Glu)、天冬氨酸(Asp)、γ-氨基丁酸(GABA)含量明显增加(P<0.01),谷氨酰胺(Gln)含量在缺血时降低,再灌注后增加(P<0.01)。灯盏花素组,ATP、Na+-K+-ATP酶和Ca2+-ATP酶含量高于脑缺血组(P<0.01)。Glu、Asp、GABA含量明显低于脑缺血组(P<0.01),Gln含量高于脑缺血组(P<0.01)。结论灯盏花素明显增加脑缺血及再灌注后ATP含量和ATP酶活性,降低兴奋性氨基酸释放,可减轻脑缺血再灌注损伤。Objective To study the effects of erigeron on ATP content, ATPase activity and the release of excitatory amino-acids of hippocampus after cerebral ischemia/reperfusion injury in gerbils. Methods To prepare the model of forebrain ischemia/reperfusion in gerbils and the insult time was 10 rain. 90 gerbils were divided into 3 groups： sham operation group, cerebral ischemia/reperfusion group,erigeron＋cerebral ischemia/reperfusion group. ATP content,ATPase activity and the content of excitatory amino-acids were assayed. Results The ATP content as well as the activities of Na^＋- K^＋ ATPase and Ca^2＋-ATPase was evidently decreased, while the content of glutamic acid（Glu）, aspartic acid （Asp）, GABA was significantly increased after cerebral ischemia （P〈0.01）. The glutamine（Gin） content was significantly decreased after cerebral ischemia, and significantly increased after reperfusion（P〈0. 01）. The ATP content and ATPase activities in erigeron group were evidently higher than those in ischemia/reperfusion group（P〈0.01）. The content of Glu and Asp and GABA in the erigeron group were significantly lower than that in cerebral ischemia group（P〈0.01）, while the Gln content was significantly higher（P〈0. 01）. Conclusion Erigeron can increase ATP content and ATPase activity after cerebral ischemia/reperfusion in gerbils, and can inhibit the release of excitatory amino-acids,which might be one of the mechanisms for erigeron to alleviate ischemia/reperfusion injury.

关 键 词：灯盏花素 缺血再灌注损伤 沙土鼠 大脑海马 ATP 含量检测 兴奋性氨基酸 神经细胞 

分 类 号：R743.3[医药卫生—神经病学与精神病学]