苯丙氨酸脱氨酶发酵工艺及其酶学性质研究  

Study of Fermentation and Properties of Phenylalanine Deaminase

作　　者：贾晓娟[1] 郭丽芸[1] 刘毅[1] 焦庆才[1] 

机构地区：[1]南京大学医药生物技术国家重点实验室,江苏南京210093

出　　处：《精细化工》2005年第11期827-830,共4页Fine Chemicals

基　　金：国家技术创新基金(02CJ-13-01-16)~~

摘　　要：选育出一株具有较高苯丙氨酸脱氨酶活性的菌株巨大芽孢杆菌AS1.127-NJU10。考察了该菌的发酵产酶条件,结果表明:蔗糖为最佳碳源、酵母浸膏和NH4C l组成最佳氮源,其质量浓度分别为:ρ(蔗糖)=20 g/L,ρ(酵母浸膏)=2 g/L和ρ(NH4C l)=10 g/L;发酵培养基最适pH=6.5,培养温度为37℃;诱导物ρ(L-苯丙氨酸)=1 g/L时,酶活最高达1 070 U。同时对苯丙氨酸脱氨酶的性质进行了研究,结果表明:该酶最适pH=5.8,最适温度为40℃,反应液中添加φ(吐温-80)=0.2%和c(K+)=10-5mol/L能明显提高酶活。The strain of Bacillus magterium AS1. 127 - NJU10 was firstly selected with high phenylalanine deaminase activity. Fermentation conditions for phenylalanine deaminase were studied. The result showed that,20 g/L saccharose was the best carbon sources,2 g/L yeast extract and 10 g/L NH4Cl were the best nitrogen sources, the optimal temperature and the initial pH of the culture medium for enzyme production were 37℃ and 6. 5 respectively, and the concentration of inducer L- phenylalanine was 1 g/L. The highest enzyme activity of 1 070 U was achieved under these optimal conditions. In addition, the properties of deaminase were studied, the optimal temperature and pH being 40℃ and 5.8 respectively. Enzyme activity was frection remarkably enhanced in the presence of volume 0. 2% Tween-80 and 10^-5 mol/L K^＋.

关 键 词：苯丙氨酸脱氨酶 巨大芽孢杆菌AS1.127-NJU10 发酵 

分 类 号：O93[理学]