灯盏细辛对NMDA所致大鼠视网膜神经元损伤的保护作用(英文)  

Neuroprotective effects of Erigeron Breviscapus (vant) Hand-Mazz on NMDA-induced retinal neuron injury in the rats

作　　者：石晶明[1] 蒋幼芹[1] 刘旭阳[2] 

机构地区：[1]中南大学湘雅二医院眼科,中国湖南省长沙市410011 [2]美国威斯康辛大学医学院眼科

出　　处：《国际眼科杂志》2005年第5期859-863,共5页International Eye Science

摘　　要：目的:已有一些临床试验和基础研究显示灯盏细辛(GerigeronBreviscapus(vant)Hand-Maz2EBHM),对青光眼患者及动物模型有神经保护的作用,本研究探讨灯盏细辛是否对NMDA导致的大鼠RGCL神经元兴奋毒性有保护作用。方法:60只健康SD大鼠随机分为4组,其中6只为正常对照组(A组),其余54只随机分为3组,分别为B组(EBHM组),C组(生理盐水+NMDA组),D组(EBHM+NMDA组),每组各18只大鼠。C、D两组大鼠右眼玻璃体内注射NMDA10nmol/2μL制成视网膜损伤模型,左眼玻璃体内注射相同剂量PBS液作为自身对照。B组及D组均在NMDA注射前7d起按150mg/(kg·d)日剂量予60g/LEBHM腹腔内注射,C组予生理盐水0.5mL腹腔内注射。在NMDA处理后4,7,14d处死动物剥取视网膜作全层铺片行RGCL神经元计数分析。结果:正常对照组双眼RGCL神经元计数比较差异无显著性意义(P=0.200)。NMDA干预后4,7,14dEBHM组RGCL神经元计数,双眼与正常对照组比较差异无显著性意义(P>0.05)。生理盐水+NMDA及EBHM+NMDA组实验眼在以上各时段RGCL神经元计数与正常组比较差异均有非常显著性意义(P<0.001),左眼与正常对照组比较差异无显著性意义(P>0.05)。实验眼14d时RGCL神经元计数EBHM+NMDA组高于生理盐水+NMDA组,二者比较差异有显著性(P=0.044),但仍低于正常对照组(P<0.05)。结论:单独使用EBHM对正常大鼠RGCL神经元计数无影响,EBHM可对NMDA所致大鼠RGCL神经毒性提供部分保护作用。AIM： To investigate whether Erigeron Breviscapus （vant） Hand-Mazz （EBHM） EBHM has neuroprotective effect against N-methyl-D-aspartate （NMDA）-induced neuron death in retinal ganglion cell layer （RGCL）. METHODS： 60 healthy SD rats were randomly divided into four groups. 6 animals were normal control group （group A）. The others were divided as group B （EBHM group）, group C （normal saline＋NMDA group） and group D （EBHM ＋ NMDA group）. Each group had 18 rats. 10nmol NMDA was intravitreally injected to induce partial damage of the neurons in RGCL in the right eyes of Groups C and D. Same volume PBS was intravitreally injected into the left eyes as self-control. Groups B and D were pre-treated intraperitoneally with 6g/L EBHM solution at a dose of 150mg/kg body weight/day seven days before and after NMDA treatment. Group C were administrated intraperitoneally with 9g/L normal saline at the same time of EBHM injection. Rats were sacrificed at 4, 7,14d after NMDA treatment. Flat whole retinas were stained with 5g/L cresyl violet and neuron counting in RGCL from both eyes were observed. Each subgroup had 6 rats. RESULTS： There was no significant difference of neu- ron counting in RGCL between the right eye and the left eye in group A （P=0.200）. There was no significant difference between normal control group and EBHM group either in the right eyes or in the left eyes at 4, 7 and 14 d respectively after intravitreal injection of 10nmol NMDA in group C and group D. （P=0.636, P=0.193）. Neuron counting of RGCL in group C and D was significantly decreased in the NMDA-treated eyes at 4, 7 and 14d after intravitreal injection （P〈0.001）. There was no significant difference between self-control eyes group and nor- mal control group（P〉0.05）. However, neuron counting was significantly higher in the EBHM＋NMDA group than normal saline ＋NMDA group at 14days after intravitreal injection （P=0.044）, but was lowered than normal control group （P〈0.05）. CONCLUSION：

关 键 词：兴奋性氨基酸 青光眼 神经保护 灯盏细辛 部分保护作用 NMDA SD大鼠 神经元损伤 视网膜 玻璃体内注射 

分 类 号：R285.5[医药卫生—中药学]