刺五加联合尼莫地平对兔实验性急性青光眼视网膜MDA,SOD,GSH含量的影响  

The effect of acathopanax senticosus and nimodipine on content of MDA/SOD/GSH of retina in rabbit eyes with experimental acute glaucoma

作　　者：陆志荣[1] 王权[1] 陈辉[1] 管怀进[1] 

机构地区：[1]南通大学附属医院眼科中心,中国江苏省南通市226001

出　　处：《国际眼科杂志》2005年第5期907-910,共4页International Eye Science

基　　金：中国江苏省中医药管理局资助(No.2001-108)

摘　　要：目的:探讨中药刺五加(acathopanaxsenticosu,sAS)和钙通道阻滞剂尼莫地平(nimodipine)对青光眼视网膜中丙二醛(maleicdialdehyde,MDA)、超氧化物歧化酶(superoxidedismutase,SOD)、谷胱甘肽(glutathion,eGSH)含量的影响。方法:通过前房平衡盐溶液持续灌注建立日本大耳白兔双眼实验性急性青光眼模型,动态测定青光眼兔眼视网膜中MDA,SOD,GSH的动态变化。然后,将实验兔随机分为刺五加组、尼莫地平组、联合用药组和生理盐水组,每组5只,分别于高眼压结束前2￣3min静脉注射药物。高眼压结束后24h处死动物,测定视网膜组织中MDA,SOD,GSH的变化。结果:高眼压后兔视网膜组织中MDA含量增高,SOD活性下降和GSH含量下降。使用药物后,AS组,尼莫地平组,联合用药组的MDA含量分别为7.015±0.344,8.021±0.385,6.561±0.228nmol/mgprot,而生理盐水组为9.009±0.276nmol/mgprot,两者相比,用药后MDA含量明显下降(P<0.01);SOD活性在上述用药组中分别为69.898±2.089,61.416±2.369,74.491±3.254u/mgprot,同生理盐水组50.732±2.880U/mgprot相比,活性上升(P<0.01);GSH含量在上述用药组中分别为60.354±1.625,58.548±1.953,61.938±1.814mg/gprot,较生理盐水组的54.360±1.585mg/gprot亦有明显增加(P<0.01)。结论:实验性青光眼兔眼自由基产生增多;刺五加和尼莫地平能够抑制视网膜自由基的产生,提高视网膜的抗氧化能力,有望临床用于青光眼视神经保护的治疗。AIM： To study the effect of acathopanax senticosus （AS） and nimodipine on content of MDA/SOD/GSH of retina in rabbit eyes with experimental acute glaucoma. METHODS： The rabbit models of experimental acute glaucoma were prepared by perfusing balanced salt solu- tion into anterior chambers. The dynamic curves of MDA/SOD/GSH were detected. According to the results, The rabbits were divided into AS group, nimodipine group, united group and saline group. The medcine was injected respectively into veins of the rabbits before the end of perfusion. MDA, SOD and GSH were examined at 24h post-perfusion. RESULTS： Compared with saline group, the content of GSH was increased significantly from 54.360 ± 1.585mg/gprot to 60.354±1.625mg/gprot in AS group, 58.548 ±1.953mg/gprot in nimodipine group, 61.938 ± 1.814mg/gprot in united group （P〈0.01）. So is the activity of SOD, from 50.735±2.880U/mgprot to 69.898 ±2.089, 61.416 ±2.369 and 74.491 ±3.254u/mgprot （P 〈0.01）. whereas the content of MDA was decreased from 9.009±0.276nmol/mgprot to 7.015±0.344, 8.021±0.385, 6.561± 0.228nmol/mgprot （P〈0.01）. CONCLUSION： Free radical concentration in experimental glaucomatous eye was elevated significantly. Both AS and nimodipine could inhibit the free radical and enhanced anti-oxidative activity induced by experimental glaucoma in rabbit. Two drugs may be applied to the prevention of optic nerve in glaucoma.

关 键 词：刺五加 尼莫地平 丙二醛 超氧化物歧化酶 谷胱甘肽 实验性青光眼 兔 日本大耳白兔 GSH含量 MDA含量 SOD活性 眼视网膜 尼莫地平 青光眼 刺五加 实验性 

分 类 号：R775[医药卫生—眼科]