大鼠激素性白内障中Na^+-K^+ATP酶活性的变化  

Investigation of Na^+-K^+ATPase activity in steroid-induced cataract

作　　者：王建伟[1] 严宏[1] 刘兵[2] 哈文静[1] 王永强[1] 丁正华[1] 

机构地区：[1]第四军医大学唐都医院眼科,中国陕西省西安市710038 [2]第四军医大学唐都医院实验中心,中国陕西省西安市710038

出　　处：《国际眼科杂志》2005年第5期919-921,共3页International Eye Science

基　　金：中国军队医学杰出人才基金资助项目(No.04J011)

摘　　要：目的:观察激素性白内障中Na+-K+ATP酶活性变化,探讨激素性白内障发病机制。方法:大鼠的96只透明晶状体随机分为对照组(DMEM)、地塞米松诱导的白内障组(DMEM+地塞米松10μmol/L),体外培养7d,动态观察晶状体混浊情况,1,3,5,7d分别从各组取12只晶状体,测定晶状体中的Na+-K+ATP酶活性。结果:7d对照组晶状体呈雾状混浊,白内障组晶状体出现重度核混浊。白内障组培养3,5,7d,Na+-K+ATP酶活性分别下降约23.5%(P=0.002),49.6%(P<0.001),75.8%(P<0.001),而对照组Na+-K+ATP酶活性下降无显著性意义。结论:地塞米松可诱导离体大鼠晶状体产生核性白内障,离子转运障碍可能参与激素性白内障的形成。AIM： To observe Na^＋-K^＋ATPase activity in dexamethason-induced organ-cultured rat lens, and to study the mechanisms of steroid cataract. METHODS： Ninety-six lenses cultured in vitro were divided into 2 groups randomly： control group （DMEM） and dexamethason-induced cataract group （DMEM＋dexamethason 10μ mol/L）. Seven days after culture, the opacities of lenses were evaluated daily using a dissecting microscope. On 1, 3, 5, 7 and 12d, lenses of each group were homogenized and Na^＋-K^＋ATPase activity was measured. RESULTS： Lenses of control group exhibited mist-like opacity, while the cataract group showed dense nuclear opadty on 7d. In cataract group, Na^＋-K^＋ATPase activity decreased 23.5% （P=0.002）, 49.6% （P〈0.001）, 75.8% （P〈0.001） on 3, 5 and 7d, respectively. However, there was no significant change in Na^＋-K^＋ATPase activity in control group. CONCLUSION： Dexamethason can induce nuclear cataract in cultured rat lens. Ion transport obstacle may take part in steroid-induced cataract formation.

关 键 词：糖皮质激素 白内障 NA^+-K^+ATP酶 Na^+-K^+ATP酶活性 激素性白内障 大鼠晶状体 透明晶状体 晶状体混浊 地塞米松 体外培养 核性白内障 对照组 

分 类 号：R776.1[医药卫生—眼科]