Spectrophotometric and Voltammetric Studies on the Interaction of Heparin with Phenosafranine  

作　　者：Wei SUN Ya Qin DING Kui JIAO 

机构地区：[1]College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 [2]Key Laboratory of Rubber-Plastics of Ministry of Education, Qingdao University of Science and Technology, Qingdao 266042

出　　处：《Chinese Chemical Letters》2005年第11期1515-1518,共4页中国化学快报（英文版）

基　　金：supported form the National Natural Science Foundation of China(20405008,20375020);the Natural Science Foundation of Qingdao City(04-2-JZ-114);the Doctoral Foundation of Qingdao University of Science and Technology(0022125).

摘　　要：The interaction of phenosafranine （PSF） with a glycosaminoglycans of heparin （Hep） in aqueous solution has been characterized by UV-Vis absorption spectrophotometry and cyclic voltammetry in pH 1.5 Britton-Robinson （B-R） buffer solution. The addition of Hep caused decrease of the absorbance of PSF at 532 nm and the redox peak current of PSF. The study showed that an supramolecular complex of PSF-Hep was formed because of the electrostatic attraction of negatively charged Hep with the positively charged PSF, which resulted in the decrease of the equilibrium concentration of PSF in solutions, and the decrease of the absorbance or the peak current of PSF. The stoichiometry of the Hep/PSF complex was further calculated by voltammetric data with the result of 1：1 complex.The interaction of phenosafranine （PSF） with a glycosaminoglycans of heparin （Hep） in aqueous solution has been characterized by UV-Vis absorption spectrophotometry and cyclic voltammetry in pH 1.5 Britton-Robinson （B-R） buffer solution. The addition of Hep caused decrease of the absorbance of PSF at 532 nm and the redox peak current of PSF. The study showed that an supramolecular complex of PSF-Hep was formed because of the electrostatic attraction of negatively charged Hep with the positively charged PSF, which resulted in the decrease of the equilibrium concentration of PSF in solutions, and the decrease of the absorbance or the peak current of PSF. The stoichiometry of the Hep/PSF complex was further calculated by voltammetric data with the result of 1：1 complex.

关 键 词：HEPARIN PHENOSAFRANINE interaction VOLTAMMETRY spectrophotometry. 

分 类 号：O629.1[理学—有机化学]