缺氧对肺动脉内皮细胞MMP-2、MMP-9表达的影响  

EFFECT OF HYPOXIA ON MMP-2 AND MMP-9 EXPRESSION IN PULMONARY ARTERY ENDOTHELIAL CELLS

作　　者：叶红[1] 马万里[2] 曹福元[1] 柯丹[1] 刘声远[1] 王迪浔[1] 

机构地区：[1]华中科技大学同济医学院病理生理系,卫生部呼吸系疾病重点实验室,武汉430030 [2]华中科技大学同济医学院附属协和医院呼吸内科,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2005年第5期511-514,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金重点项目(39730190)

摘　　要：目的观察缺氧对培养的猪肺动脉内皮细胞基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)的表达的影响。方法采用RT-PCR、Western blot、底物胶电泳(酶谱图)、免疫细胞化学等方法。结果肺动脉内皮细胞缺氧24h可使MMP-2的mRNA表达和蛋白分泌减少,酶活性减弱,与常氧组比较有显著性差异,MMP-9无明显变化。结论缺氧时MMP-2降低,溶解细胞外基质的能力降低,可能是缺氧性肺血管构型重组的机制之一。Objective To investigate the effect of hypoxia on the expression of MMP-2 and MMP- 9 in pulmonary arterial endothelial cells. Methods RT-PCR, gelatin zymography, western blot, and immunocytochemistry were used. Results After exposure to hypoxia for 24h, the expression of MMP-2 mRNA and protein, as well as the activity, decreased significantly. However, MMP-9 expression was not affected by hypoxia. Conclusion Hypoxia can induce the reduction of MMP-2 expression, which may lead to a decrease in matrix-degradation and contribute to the mechanism of hypoxic pulmonary vascular remodeling.

关 键 词：缺氧 肺动脉内皮细胞 基质金属蛋白酶 

分 类 号：R363[医药卫生—病理学]