Regulation of Survivin and CDK4 by Epstein-Barr virus encoded latent mem-brane protein 1 in nasopharyngeal carcinoma cell lines  

作　　者：Mi Dan AI Li Li LI Xiao Rong ZHAO Yong WU Jian Ping GONG Ya CAO 

机构地区：[1]Cancer Research Institute, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan410078, China [2]Molecular Medical Center, Tongji Hospital, Tongji Medical University, Wuhan 430030, China

出　　处：《Cell Research》2005年第10期777-784,共8页细胞研究（英文版）

基　　金：National Nature Science Foundation for Distinguished Young Scholar of China (No.39525022);National Basic Research Program(No.2004CB518703); National Nature Science Foundation of China (No.30570085).

摘　　要：Latent membrane protein 1 （LMP1）, an important protein encoded by Epstein Barr virus （EBV）, has been implied to link with the pathogenesis of nasopharyngeal carcinoma （NPC）. Its dual effects of increasing cell proliferation and inhibiting cell apoptosis have been confirmed. In this study, we showed that the expression of Survivin and CDK4 protein in CNE-LMP1, a LMP1 positive NPC epithelial cell line, is higher than in LMP1 negative NPC epithelial cell line- CNE1, and the expression is LMP1 dosage-dependent. Although it was reported that Survivin specifically expressed in cell cycle G2/M phase, our studies suggested that LMP1 could promote the expression of Survivin in G0/G1, S and G2/ M phase. It also showed that Survivin and CDK4 could be accumulated more in the nuclei triggered by LMP1. More interestingly, Survivin and CDK4 could form a protein complex in the nuclei of CNE-LMP1 rather than in that of CNE1, which demonstrated that the interaction between these two proteins could be promoted by LMPI. These results strongly suggested that the role of LMP1 in the regulation of Survivin and CDK4 may also shed some light on the mechanism research of LMP1 in NPC.

关 键 词：EBV latent membrane protein 1 cell cycle G1/S check point SURVIVIN CDK4. EBV latent membrane protein 1 cell cycle G1/S check point SURVIVIN CDK4. 

分 类 号：R739.63[医药卫生—肿瘤]