重组Endostatin基因治疗大鼠胶质瘤及其微血管密度改变  

Recombinant endostatin gene therapy for rat glioma and intratumoral microvessel density change

作　　者：蔺玉昌[1] 苗增利[1] 张敬宁[2] 缪以峰[1] 孙继勇[1] 黄红雨[1] 张熔熔[3] 唐威[3] 王丽君[3] 

机构地区：[1]江苏省无锡市第二人民医院神经外科,214002 [2]江苏省无锡市第二人民医院保健科,214002 [3]江苏省无锡市第二人民医院病理科,214002

出　　处：《中华神经医学杂志》2005年第10期991-993,共3页Chinese Journal of Neuromedicine

摘　　要：目的探讨重组Endostatin基因(EScDNA)对大鼠胶质瘤的治疗效果及肿瘤微血管密度的变化。方法对质粒包埋的Endostatin基因(pSecTagA-EScDNA)测序鉴定后,转染人脐静脉内皮细胞(ECV-304),验证Endostatin基因对血管内皮细胞增殖的抑制作用;以C6胶质瘤细胞植于SD大鼠腋部皮下制作胶质瘤模型,将pSecTagA-EScDNA直接瘤内注射。分别观察记录肿瘤体积,绘制肿瘤生长曲线;对肿瘤标本进行微血管标记、计数,并比较治疗前后肿瘤微血管数量的变化。结果重组Endostatin基因治疗后的肿瘤生长明显受抑制,使肿瘤退缩至休眠甚至完全消失;肿瘤微血管密度明显低于未治疗者。结论重组Endostatin基因通过抑制肿瘤血管新生对大鼠胶质瘤有良好的治疗效果。Objective To explore the inhibitory role of recombinant endostatin （ES） gene on the growth of rat C6 glioma and the change of intratumoral microvessel density （MVD） after endostatin gene therapy. Methods After the sequence of EScDNA in the pSecTagA-EScDNA was identified correctly, human umbilical vein endothelial cells （ECV-304） were transfected with pSecTagA-EScDNA and pSecTagA by the lipofection method to assess the suppressive effect on the growth of ECV-304. Rat C6 glioma models were established by subcutaneously implanting C6 cells in axillary fossa. PSecTagA-EScDNA was injected intratumorally. The tumor volume change was observed by drawing a tumor growth curve and the intratumoral MVD before and after gene therapy was compared. Results EScDNA and ES were detected in the transfected cells by RT-PCR and Western Blot. FCM and MTT suggested that cell cycle was prolonged and cell activity decreased in test group compared with that in control group. The tumor growth curve showed that the tumor growth was markedly suppressed in test group;the tumor MVD in ES-treatment group is lower than in control group. Conclusion EScDNA can remarkably inhibit the growth of the C6 glioma by inhibiting the glioma angiogenesis.

关 键 词：重组 ENDOSTATIN基因 神经胶质瘤 血管新生 基因治疗 

分 类 号：R73-3[医药卫生—肿瘤]