重组人纤溶酶原k1-3基因转染对血管内皮细胞的作用研究  

The study on the effect in endothelial cells of recombinant human Plasminogen k1-3 gene by polycationic liposome-mediated transfection

作　　者：陆幸妍[1] 罗利琼[1] 

机构地区：[1]广东药学院生命科学与生物制药学院,广东广州510240

出　　处：《广东药学院学报》2005年第5期580-582,579,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省医学科学技术研究基金立项(A2003338)

摘　　要：目的观察脂质体介导人纤溶酶原k1-3基因转染人脐静脉内皮细胞导致细胞凋亡和移动抑制的效应,初步探讨k1-3基因的抗血管生成机制。方法以阳离子脂质体介导人纤溶酶原k1-3基因真核表达载体pcDNA-k13转染体外培养人脐静脉内皮细胞,经细胞凋亡检测和移动抑制试验,比较对照组和实验组的凋亡率和抑制率。结果转染pcDNA-k13组有明显的细胞凋亡效应和移动抑制。结论人纤溶酶原k1-3基因在人血管内皮细胞表达,并可能通过诱导细胞凋亡及抑制细胞迁移而抑制肿瘤新生血管的生成。Objective To observe human plasminogen k1-3 gene induces apoptosis and migration inhibition in cultured ECV cells by polycationic liposome-mediated transfection. Methods human plasmlnogen k1-3 gene was recombined into pcDNA-k13, and was induced into ECV cell by polycationic liposome-mediated transfection. Cellular apoptosis assay and migration inhibition assay were carried out in treatment and control group. Results The apoptosis rate and migration inhibition rate of ECV in treatment group were both significantly improved. Conclusion It was indicated that k1-3 could be expressed in human endothelial cell, and it might inhibit the angiogenesis by inducing apoptosis and inhibiting cellular migration.

关 键 词：人纤溶酶原k1-3基因 人脐静脉内皮细胞 细胞凋亡 细胞迁移抑制 

分 类 号：R73-3[医药卫生—肿瘤]