小鼠哮喘模型的建立及其相关机理初步研究  

Establishment of Allergic Asthma Model in Mice and Expression of OX40 in the Lung

作　　者：黄莉[1] 胡玉敏[2] 陈煦艳[1] 施勤[2] 季伟[1] 

机构地区：[1]苏州大学附属儿童医院呼吸科,苏州215003 [2]苏州大学生命科学学院,苏州215007

出　　处：《实验动物与比较医学》2005年第3期140-142,146,192,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的通过制备BALB／c小鼠过敏性哮喘模型,研究共刺激分子OX40在哮喘中的表达。方法腹腔注射卵蛋白致敏小鼠,两周后雾化吸入卵蛋白激发哮喘发作,以生理盐水腹腔注射及雾化吸入为空白对照。比较支气管肺泡灌洗液(BALF)中细胞总数及嗜酸性粒细胞和淋巴细胞数百分数;肺组织HE及PAS染色以确定动物造模是否能模拟哮喘的病理状态;肺组织冰冻切片行免疫组化检测哮喘小鼠肺组织OX40的表达情况。结果卵蛋白激发后,小鼠的支气管肺泡灌洗液中的细胞总数及嗜酸性粒细胞和淋巴细胞百分数较对照组增高,且差异有统计学意义,哮喘小鼠肺组织中OX40的表达明显增多。结论 BALB／c小鼠是制备过敏性哮喘的理想模型,哮喘时小鼠肺组织中OX40的表达明显增多。Objective To establish allergic asthma model in BALB/c mice for investigating the expression of OX40 in asthma. Methods Mice were sensitized by i.p. injection of OVA ,two weeks late mice were challenged with aerosolized OVA ,saline was administered to the mice of the control group in a similar manner. Total number of bronchoalveolar lavage cells and the percents of eosinophils, lympho- cytes were counted; Part of the lung tissues were stained by HE and PAS to show if the murine model can reflect the pathological changes of asthma, and the another part of lung tisuuses were stained by immunohistology to evaluate the expression of OX40. Results Total number of cells and the percents of eosinophils lymphocytes in the BALF were increased in the model group than that of the control group, the difference was statistically significant ; the expression of OX40 in model group were more manifest than that of the control group. Conclusion The BALB/c mice were ideal animals to establish the model of allergic asthma, the expression of OX40 was increased in the lung of mice in asthma.

关 键 词：小鼠 过敏性哮喘 模型 OX40 

分 类 号：R-332[医药卫生]