血管内皮生长因子和血小板凝集素-1在膀胱癌中的表达  

Expression of vascular endothlial growth factor and throbospondin-1 in bladder cancer

作　　者：张冰[1] 黎玮[2] 蔡文清[2] 李刚[2] 

机构地区：[1]石家庄市中心医院泌尿外科,河北石家庄市050011 [2]河北医科大学第二医院泌尿外科,河北石家庄市050011

出　　处：《中国康复理论与实践》2005年第10期808-810,共3页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的观察血管内皮生长因子(VEGF)与血小板凝集素-1(TSP-1)在不同分级的膀胱肿瘤中的表达.方法 2000年4月～2003年9月的70例膀胱肿瘤患者切除标本,其中G1期28例、G2期27例、G3期15例(采用WHO病理分级),采用免疫组化法对标本进行染色,VEGF和TSP-1的表达均采用平均血管密度(MVD)表示,SPSS 10.0软件进行统计学分析,然后采用免疫荧光双染判断共表达,用激光扫描共聚焦显微镜观察.结果 VEGF表达的MVD值与肿瘤分级呈正相关;G1与G2期有非常显著性差异( P＜0.01),G2期与G3无显著性差异( P＞0.1);TSP-1表达的MVD值随着肿瘤分级增加而降低,但无相关关系,但G1期与G2、G3期有非常显著性差异( P＜0.01),G2期与G3无显著性差异( P＞0.1);在G1期,TSP-1的表达与VEGF的表达无相关关系( rs=0.167, P＞0.1);激光扫描荧光双染显示,VEGF与TSP-1可以同时表达.结论 VEGF的表达随膀胱肿瘤分级增加而增强;VEGF是促进肿瘤形成的因素;TSP-1是抑制肿瘤血管形成的因素,但其抑制作用仅发生在初期;荧光双染仅可提供共同表达的证据,不能判断表达的高低.Objective To observe the expression of vascular endothlial growth factor （VEGF） and throbospondin-1 （TSP-1） in different grade bladder cancer. Methods Specimens of 70 cases of bladder transitional cell cancer including Grade Ⅰ 28 cases, Grade Ⅱ 27 cases, Grade Ⅲ 15 cases （according to WHO pathological grade） were stained with immunohistochemistry method. The expressing values of VEGF and TSP-1 were calculated by mean vascular density （MVD）. All data was analyzed with SPSS 10, 0, and with expression of VEGF and TSP-1 was detected with immunohistochemisrty double staining. Finally, the result of expression was observed by laser scan with focus microscope. Results There was a positive correlation between the cancer grade and the expression of VEGF, and the expression of Grade Ⅰ was significant difference with that of Grade Ⅱ （P 〈0.01）, but there was no difference between Grade Ⅱ and Grade Ⅲ （ P 〈0.1）. Expression of TSP-1 decreased with grade increasing but not showing a negative correlation, while, Grade Ⅰ was significant difference with that of Grade Ⅱ and Grade Ⅲ （ P 〈0. 01）, Grade Ⅱ was not differcnt with Grade Ⅲ （ P 〈0. 1）. In 28 Grade Ⅰ cases, values of MVD of VEGF and TSP-1 had no correlation （r s =0. 167, P 〉0. 1）. Double immunostaining detecting with laser scan with focus microcope indicate showed that VEGF and TSP-1 appeared in same bladder cancer specimen. Conclusion Expression of VEGF is gradually increased from Grade Ⅰ to Grade Ⅲ , and VEGF is a promoting factor of angiogenesic bladder cancer. Expression of TSP-1 is the strongest in Grade Ⅰ cases, and TSP-1 is an inhibiting angiogenesic factor, which inhibiting function only in early stage. Immunofluorescent staining can only provide evidence of together expression of VEGF and TSP-1, cannot evaluate the degree of expression.

关 键 词：膀胱癌 血管内皮生长因子(VEGF) 血小板凝集素-1(TSP-1) 免疫组化法 平均血管密度 免疫荧光双染 激光扫描共聚焦显微镜 

分 类 号：R737.14[医药卫生—肿瘤]