p33^(ING1)和wt-p53转染对胃癌细胞株生长抑制及凋亡的作用研究  

The research of p33^(ING1),wt-p53 growth suppressing and collapsing effect toward stomach cancer cell strain

作　　者：吴福荣[1] 丁厚中[2] 冯堃[1] 李海[3] 郑斯杰[1] 倪灿荣[4] 于观贞[4] 

机构地区：[1]江苏省昆山市第一人民医院消化科,215300 [2]江苏省昆山市第一人民医院普外科,215300 [3]江苏省昆山市第一人民医院病理科,215300 [4]长海医院病理科,上海200000

出　　处：《肿瘤研究与临床》2005年第5期320-323,共4页Cancer Research and Clinic

摘　　要：目的探讨新型抑癌基因p33ING1真核表达质粒在胃癌细胞株中的表达及对胃癌细胞株的生长抑制、凋亡的作用,探索新型肿瘤治疗措施与方法。方法构建PCDNA3/p33ING1真核表达质粒,将p33ING1和wt-p53单、共染至人胃癌细胞,研究p33ING1与wt-p53间协同功能及对胃癌细胞产生的作用。结果重组PCDNA3/p33ING1真核表达质粒构建成功,经p33ING1和wt-p53单、共染的人胃癌细胞株SSCG-7901生长速度减慢,融合时间变长,周期S期变短,G0/G1期延长,凋亡增加。结论p33ING1除本身具有抑癌功能及生物活性外,同时参与p53基因并与其一起调控胃癌细胞的生长,诱导细胞的凋亡,使细胞周期阻滞,二者为相互依赖协同作用。Objective To discuss the growth suppressing, apoptosing effect of new type tumor-supressor gene-p33^ING1 in stomach cancer cell strain, and to explore new strategies and methods in tumor therapy. Methods The PCDNA3/p33^ING1 nuclear expressing microsome was constructed, p33^ING1 and wt-p53 were implanted to human stomach cancer cell both and to evaluate the effect of p33^ING1 and p53 toward stomach cancer cell and synergism between them. Results The PCDNA3/p33^ING1 nuclear expressing microsome was successfully constructed. The human stomach cancer cell strain SSCG-7901 under implantation of p33^ING1 and wt-p53 showed a significant decrease in cell growth, the coupling time was delayed, DNA synthetic phase was shortened and G0/G1 phase prolonged. The cell collapse increased. Conclusions Despite of the tumor-inhibiting effect and biochemical activation of p33^ING1 it also plays a role with p53 gene in controling growth of stomach cancer cell, inducing cell collapse and hampering cell proliferation cycle. P33^ING1 and p53 are synergistic to each other.

关 键 词：P33^ING1 WT-P53 胃癌细胞株 生长抑制 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]