秦川牛及其利秦杂种牛IGFBP3基因PCR-SSCP多态性研究  

PCR-SSCP Polymorphisms of IGFBP3 Gene in Qinchuan Cattle and Limousin×Qinchuan Cattle

作　　者：刘波[1] 陈宏[1] 蓝贤勇[1] 雷初朝[1] 张争锋[1] 

机构地区：[1]西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《中国农学通报》2005年第9期10-11,共2页Chinese Agricultural Science Bulletin

基　　金：国家863计划项目(2003AA243051);国家自然科学基金(30471238)资助

摘　　要：以IGFBP3基因作为侯选基因,对秦川牛及其利秦杂种牛群体共计114头个体IGFBP3位点进行多样性分析。结果发现,秦川牛(Q)及其利秦牛(LQ)群体IGFBP3基因座的等位基因A/B频率分别为:0.6667/0.3333和0.6829/0.3171;从位点间杂合度(He)来看,LQ>QQ;位点间有效等位基因数(Ne):QQ>LQ位点间Shannon信息熵SQQ>LQ位点间多态信息含量PICQQ>In the research, DNA sample from 114 Qinchuan cattle and Qinchuanx Limousin hybrids were analyzed on IGFBP3 exon2 with PCR-SSCP. This locus showed that three genotypes, namely AA,BB and AB ,after electrophoresis. Frequencies of the allele A and B were 0.6667/0.3333 and 0.6829/0.3171 in QQ and LQ populations, respectively. This polymorphic locus of IGFBP3 gene was at Hardy-Weinberg equilibrium （P〉 0.05）.In He, LQ〉QQ, and in Ne, Shannon index, PIC, QQ〉LQ.

关 键 词：秦川牛 利木赞牛 IGFBP3基因 PCR—SSCP IGFBP3 侯选基因 杂种牛 多态性研究 SSCP Shannon信息熵 

分 类 号：S825.5[农业科学—畜牧学] S828.2[农业科学—畜牧兽医]