卡介苗基因组DNA对哮喘小鼠模型过敏性气道炎症的影响  

Prevention and treatment of mice with airway allergic inflammation using bacillus Calmette-Guérin DNA

作　　者：商艳[1] 李强[1] 胡振林[2] 施柯[2] 周凤娟[2] 刘忠令[1] 孙树汉[2] 

机构地区：[1]第二军医大学长海医院呼吸内科,上海200433 [2]第二军医大学医学遗传学教研室

出　　处：《中国呼吸与危重监护杂志》2005年第5期352-354,347,i0001,共5页Chinese Journal of Respiratory and Critical Care Medicine

摘　　要：目的探讨腹腔注射卡介苗基因组DNA(BCGDNA)对哮喘小鼠模型的影响。方法BALB/c小鼠经鸡卵蛋白(OVA)免疫建立哮喘模型,分别于致敏前、激发时腹腔注射100μgBCGDNA,观察哮喘小鼠支气管肺泡灌洗液(BALF)中细胞总数和嗜酸性粒细胞数(EOS),ELISA检测脾单核细胞(SMNC)培养上清和BALF中白细胞介素4(IL4)、IL5、IL12及γ干扰素(IFNγ)的含量变化,常规病理切片观察肺内炎症情况,ABPAS染色观察杯状细胞增生和黏液分泌情况。结果哮喘小鼠OVA致敏前及激发时腹腔注射BCGDNA后可明显增加BALF和脾单核细胞培养上清中IL12和IFNγ的产生,使IL4和IL5的产生减少,BALF中嗜酸性粒细胞减少,黏液过度分泌明显减轻。结论哮喘小鼠腹腔注射BCGDNA可诱导IL12和IFNγ产生,抑制Th2型细胞反应,减轻哮喘气道炎症。Objective To investigate the eftects of intraperitoneally administered Mycobacterium bovis bacillus Cahnette Guerin（ BCG）-DNA on a routine model of asthma. Methods The asthma model was established by immunizing BALB/c mice with ovalbumin（OVA）. BCG-DNA （100μg） was intraperitoneally administered before OVA sensitization or OVA challenge.Total cell count and eosinophil （EOS） count in brochoalveolar lavage fluid （BALF） were measured. The levels of interleukin-4 （IL-4）, IL-5, IL-12, and IFN-γ in splenic mononuelear cell（ SMNC）culure supernatiant and broehoalveolar lavage fluid（BALF） were determined using Enzyme-Linked-hnmunn-Sorbent Assay （ELISA）. Pulmonary inflammation was observed on routine pathological slides and the hyperplasia and hypcrsecretion of goblet cells stained with Alcian blue/periodic acid Schiff（ AB- PAS）were also observed. Results BCG-DNA administered intraperitnneally before OVA sensitization and on OVA challenge significantly, inereased the levels of IL-12 and IFN-γ while decreased the levels of IL-4 and IL-5 in BALF and SMNC cuhtu＇e supernataut. BCG-DNA administration also reduced the amount of eosinophils in BALF,suppressed mucus produetion and allergic＇ airway inflammation. Conclusions Intrapefitoneally administered BCG-DNA induces production of IL-12 aud IFN-γ and inhibites Th2 responses resulting a suppression of allergic airway inflammation. Our findings provide a new protocol for the treatment anti prevention of asthma.

关 键 词：卡介苗 基因组DNA 哮喘 小鼠 动物模型 过敏性气道炎症 

分 类 号：R562.25[医药卫生—呼吸系统] R186[医药卫生—内科学]