免疫增强及抑制因子在鼻咽癌中的表达及意义  

Expression of Heat Shock Proteins and Cytokines in Nasopharyngeal Carcinoma and Its Significance

作　　者：程磊[1,2] 刘立思[1] 张明[1] 肖成峰[1] 周梁[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院耳鼻咽喉科,武汉430022 [2]复旦大学附属眼耳鼻喉医院耳鼻喉科 [3]复旦大学附属耳鼻喉医院耳鼻喉科,上海200031

出　　处：《中国眼耳鼻喉科杂志》2005年第5期287-289,共3页Chinese Journal of Ophthalmology and Otorhinolaryngology

摘　　要：目的检测免疫增强因子 Hsp27、Hsp70、Hsp90、IL-2、TNFα及免疫抑制因子 IL-10、TGFβ_1在鼻咽癌中的表达,探讨其在免疫治疗上的意义。方法应用 Western blot 检测 Hsp27、Hsp70、Hsp90在鼻咽癌及鼻咽炎症活检组织中的表达,用双抗体夹心酶联免疫吸附测定(enzyme linked immuno sorbent assay,ELISA)法检测鼻咽癌、鼻咽炎症及正常人血清中 IL-2、TNFα、IL-10、TGFβ_1的表达水平,了解机体的免疫状态。结果鼻咽癌组织中Hsp27较鼻咽炎症组织高表达(P<0.05),Hsp70、Hsp90表达无明显差异,鼻咽癌血清中 TNFα(P<0.01)、TGFβ_1(P<0.01)水平较正常及鼻咽炎症升高,IL-10表达明显降低(P<0.01),IL-2表达无明显变化(P=0.425)。结论根据鼻咽癌患者免疫增强及抑制因子表达情况,可以应用小剂量的 IL-2,TGFβ单克隆抗体,Hsp 多肽复合物疫苗来辅助鼻咽癌的治疗。Purpose To detect the expression of immune protective factor Hsp27, Hsp70, Hsp90, IL-2, TNFα and immune inhibitor IL-10,TGFβ1 in nasopharyngeal carcinoma （NPC） as well as to explore the potential efficacy on immune therapy. Methods The expression of Hsp27, Hsp70, HspgO in tumor and chronic nasopharyngitis tissue was studied with Western blot method. Serum level of IL-2 ,IL-10,TNFα,TGFβ1 was measured using ELISA. Immune condition of NPC patients was analysed. Results Hsp27 elevated remarkably （ P 〈 0.05 ） while HspgO and Hsp70 showed no significant difference between NPC and control tissues. Expression of serum TNFα,TGFβ1 improved highly wheras IL-10 decreased significantly in NPC compared with normal control （P 〈 0.01 ）, There is no alteration in the level of IL-2. Conclusion According to the detection results of immune protective factors and immune iubibitors, We can use little dose IL-2, TGFβ antigen, Hsppolypeptide compound vaccine in patients with nasopharyngeal carcinoma to improve therapeutic effects.

关 键 词：鼻咽癌 热休克蛋白 细胞因子 免疫治疗 鼻咽癌组织 免疫抑制因子 免疫增强 双抗体夹心酶联免疫吸附测定 BLOT检测 HSP27 

分 类 号：R739.65[医药卫生—肿瘤]