人内皮抑素基因导入对裸鼠舌鳞癌移植瘤的抑制影响  

Intra-tumoral and intra-muscular administration of human endostatin gene inhibits Tca8113 cell growth

作　　者：潘朝斌[1] 黄洪章[1] 赵小朋[1] 张彬[1] 李劲松[1] 

机构地区：[1]中山大学附属第二医院口腔颅颌面外科

出　　处：《中国口腔颌面外科杂志》2005年第3期218-222,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：广东省自然科学基金(04300248);广东省医学科学技术基金(2003A025)

摘　　要：目的:探讨经体内注入人内皮抑素(humanendostatin,hES)基因治疗口腔鳞癌的可行性。方法:荷瘤人舌鳞癌Tca8113细胞裸鼠瘤体内和肌内分别注射含hES的基因质粒,观察转入hES基因对舌鳞癌移植瘤生长的影响,免疫组织化学检测肿瘤组织中hES及血管内皮细胞生长因子(vascularvesselendotheliacellgrowthfactor,VEGF)的表达,Weidern法行肿瘤微血管密度(microvesseldensity,MVD)计数,流式细胞仪检测肿瘤细胞凋亡率,采用精确概率法和单因素方差分析进行统计学处理。结果:荷瘤注射hES后第16天,瘤体注射组抑瘤率为75.0%,肌内注射组抑瘤率为66.1%(P<0.01)。免疫组织化学检测结果显示,hES在瘤体注射组和肌内注射组肿瘤中的表达率分别为90.0%和85.0%,均高于对照组的20.0%(P<0.01)。2实验组肿瘤MVD值分别为18.60±3.44和16.70±2.63,均低于对照组的22.40±3.41(P<0.01)。流式细胞仪检测发现,瘤体注射和肌内注射hES基因质粒后,肿瘤细胞凋亡率分别为11.36%±3.20%和8.08%±2.00%,均高于对照组的1.80%±0.50%(P<0.01)。结论:注射hES基因具有抑制舌鳞癌Tca8113细胞移植瘤生长的效应。PURPOSE： To investigate the inhibitory effect of Lipofectatmin-mediated p^BLST-hES on Tca8113 cell implantation tumor growth in rivo. METHODS： To observe tumor growth, the nude mice bearing Tca8113 tumors received injection of lipofectamin-plasmid complexes, then the tumors were measured once in every three days. 16 days after injection, S-P immunohistochemistry was used to detect the expression of hES and VEGF in the tumors, FCM was used to detect the apoptosis of tumor cell. With CD34 staining, the MVD in tumors was counted. Fisher＇s exact test and the analysis of variance were adopted for statistical analysis of count data and measurement data. RESULTS： Compared with the control group, the reduction of tumor volume was about 75.0% in the group of intratumoral administration hES gene and 66.1% in the group of intramuscular injection of hES gene. The volume reduction obtained in both groups was statistically significant when compared with controls （P〈0.01）. The expression of hES was 90.0% and 85.0% respectively, both significantly higher than that in the control group （20.0%,P〈0.01）. Otherwise, the expression of VEGF in the experimental groups was lower than that in the controls （P〈0.01）. The MVD was 18.60±3.44 in tumors received injection of hES gene intratumorally and 16.70±2.63 in tumors received injection of hES gene intramuscully, both showed statistically significant when compared with that in the control group （P〈0.01）, The apoptosis rate was 11.36%±3.20% and 8.08%±2.00% respectively, both higher than that of the control group （1.80%±0.50% ,P〈0.01）. CONCLUSION： The results suggested that intratumoral and intramuscular injection of hES gene can inhibit the growth of Tca8113 cell tumor effectively.

关 键 词：内皮抑素 基因治疗 抗血管生成 舌鳞癌细胞Tca8113 

分 类 号：R739.8[医药卫生—肿瘤]