PCNA及凋亡相关基因蛋白在翼状胬肉中的表达及临床意义  

Expression and Clinicalsignifcance of Proliferating Cell Nucler Antigin and Apoptosis Gene Assoisted Protein in Pterygium

作　　者：胡乃民[1] 李海[1] 巫宇舟[1] 周欣[1] 陈芳[1] 

机构地区：[1]昆山市第一人民医院,江苏昆山215300

出　　处：《实用医技杂志》2005年第10A期2690-2692,共3页Journal of Practical Medical Techniques

基　　金：昆山市社会发展科技计划(项目编号KS0513)

摘　　要：目的:分析PCNA、Bcl-xl、bak蛋白在翼状胬肉中的表达,探讨其与翼状胬肉增殖的关系。方法:应用免疫组化法对40例翼状胬肉组织中PCNA、Bcl-xl、bak蛋白的表达进行观察和分析。结果:PCNA在翼状胬肉中的阳性表达率显著高于正常结膜(P<0.01)。Bcl-xl主要表达于翼状胬肉的上皮基底层,正常结膜无表达。bak在翼状胬肉与正常结膜中的表达差异无显著性。PCNA与Bcl-xl表达呈正相关。结论:翼状胬肉的发生、发展与PCNA和Bcl-xl、bak的异常表达有关,可能是细胞异常增殖和凋亡失衡的结果。Objective To evaluate the expression of proliferating cell nuclear antigen（PCNA）,Bcl-xl gene and bak gene products in pterygium,and study the ralationship of them with proliferating status of pterygium. Methods The immunohistochemical methods was performed in study of expression of PCNA, Bcl-xl and bak in 40 pterygial apecimens. Results The expression of PCNA in the pterygium was significantly higher than that of normal conjunctira （P〈0. 01 ）. Bcl-xl staining noted predominantly in the basal epithelial layer of cells, but normal conjunctiral specimens showed no enidence of it. there was no difference in the expression of bak between pterygium and nomal conjunctiva. The expression of PCNA was derect related to that of Bcl-xl. Conclusions The abnormal expression of PCNA and Bcl-xl may be a positive factor in the pathogenesis of pterygium. The results suggest that pterygia derelopment may result from abnormal preliferation and unbalanced apoptosis.

关 键 词：翼状胬肉 增殖 凋亡 PCNA BCL-X1 BAK 

分 类 号：R777.33[医药卫生—眼科]