海洋真菌KLEB-07培养条件的研究  

Optimal cultivation conditions of marine fungi KLEB-07

作　　者：范国涛[1] 朱天骄[1] 崔承彬[1] 顾谦群[1] 朱伟明[1] 

机构地区：[1]教育部海洋药物重点实验室中国海洋大学海洋药物与食品研究所,山东青岛266003

出　　处：《中国海洋药物》2005年第5期11-14,共4页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(30171102);国家高技术研究发展计划(863计划)项目(2001AA624020);山东省科技攻关项目(0121100107);山东省自然科学基金重点项目(Z2001C01);国家教育部长江学者奖励计划相关基金资助

摘　　要：一株海洋来源的真菌KLEB-07发酵物对小鼠乳腺癌温敏型tsFT210细胞具有细胞凋亡诱导活性。为得到该菌株产生抗肿瘤活性物质的最佳培养条件,对其发酵培养基进行了优化。并比较了不同培养基、不同发酵条件下KLEB-07发酵物的活性强弱,考察了发酵物中活性成分对温度、酸碱的稳定性及其所在的活性部位。结果表明KLEB-07在28℃,130r.min-1发酵7d产生抗肿瘤活性物质的活性最强,发酵物对高温、酸碱稳定,其活性部位在醋酸乙酯萃取液中,为研究该菌株的抗肿瘤活性成分提供了依据。It was found that the metabolites produced by a marine fungi KLEB-07 showed the induction of cell apoptosis activity on mouse tsFT210 ceils. In order to find the best fermentation condition, the optimization of fermentation medium was studied. Activities of the ferments of KLEB-07 under different fermentation media and conditions, and the stability of the ferments were investigated. The results showed that the KLEB-07 produced the highest anti- tumor bioactivity of the ferments under the conditions of 28℃ and 130 r · min^-1 for 7 days. The bioactive substances of ferments were stable and mainly existed in ethyl acetate layer. These results provide valuable informations for the further study of the antitumor active substances of KLEB-07.

关 键 词：海洋真菌 细胞凋亡诱导 抗肿瘤活性物质 发酵条件 

分 类 号：R931.711[医药卫生—生药学]