多肽研究XIX：丙型肝炎病毒的免疫选择性  

STUDIES ON PEPTDE XIX: HEPATITISC VIRUS(HCV)IMMUNE SELECTION

作　　者：刘刚[1] 梁争论[1] 蔡孟深[1] 孙涛[1] 庄辉[1] 陶其敏[1] 郭建平[1] 

机构地区：[1]北京医科大学药学院有机化学研究室,北京医科大学公共卫生学院流行病学研究室,北京军事医学科学院基础医学研究所,北京医科大学人民医院肝病研究所

出　　处：《药学学报》1996年第5期358-363,共6页Acta Pharmaceutica Sinica

基　　金：卫生部"八五"招标项目

摘　　要：丙型肝炎病毒（ＨＣＶ）基因组有显著的异源性和高度的可变性。其中Ｅ２ＨＶ（高变区）认为是宿主识别ＨＣＶ，并且产生免疫压力的靶目标之一。文中设计并合成了三段多肽抗原Ｐ２，Ｐ９和Ｐ１０。抗原性及免疫原性研究结果表明：（１）ＨＣＶ宿主体内存在抗－Ｅ２ＨＶ抗体，但这种抗体是非中和性的，或者是抗体滴度太低。不足以中和ＨＣ病毒抗原；（２）同一基因型的ＨＣＶ抗－Ｅ２ＨＶ抗体有某种共同的结构特点，从而使某一ＨＣＶ病毒株Ｅ２ＨＶ抗原能够与其它病毒株抗－Ｅ２ＨＶ抗体反应，大约有３０％的代表性；（３）丙型肝炎病毒Ｅ２ＨＶ区里碳端３９８～４１２多肽片段可以引起动物免疫应答。HCV has characteristics of rapid variablity.The amino terminus of E2/NS1 ofHCV(amino acids sequence 384～414),which is hypervariable with respect to both nucleotide andamino acid sequence,has been termed the E2 HV domain or HVR1. The E2 HV domain appears to bearapidly evolving region of the HCV genolnes which may contain linear neutralizing epitopes and theE2 HV are under immune selection, For further studies of the immunogenicity on E2 HV of HCV,weselected three peptide fragments from the full length of E2 HV region sequence and synthesized themwith SPPS method. The amino acid sequences are shown as following:P2:VDGDTHVT-GGAQAKTTNR(381～398);P9:STHVTGAVQGHSIRGTTSLFTSGPAQKIQ(384～412);P10:RTYTSGGTAGHTTSGITSLFSPGASQKIQ(384～412), From the results of ELISA and anti-peptideAbs of rabbit sera,we conclude that there are anti-E2 HV Abs in immune host but they could notneutralize HCV,so these Abs were not reactive with all E2 HV epitopes that resulted in immuneselection of escape mutants;the anti-E2 HV Abs,probably from the same genotype,contained somecommon structure in which E2 HV epitopes react with other anti-E2 HV Abs at 30%;the C-terminusof E2 HV region(398～412)caused the immune response to rabbits.

关 键 词：丙型肝炎 合成多肽 抗原性 免疫 丙型肝炎病毒 

分 类 号：R512.63[医药卫生—内科学] R373.2[医药卫生—临床医学]