GC／MS内标法测定蛋黄磷脂中花生四烯酸和二十二碳六烯酸含量  

QUANTITATIVE DETERMINATION OF ARACHIDONIC ACID AND DOCOSAHEXAENOIC ACID IN YOLK PHOSPHOLIPID OF HEN EGG BY GC/MS INTEKNAL.STANDARD METHOD

作　　者：沈晓京[1] 赖炳森[1] 汪聪慧[1] 董风霞[1] 

机构地区：[1]解放军北京医学高等专科学校生化教研室,北京公安部第二研究所仪器分析室

出　　处：《药学学报》1996年第5期394-397,共4页Acta Pharmaceutica Sinica

摘　　要：ＧＣ／ＭＳ内标法测定蛋黄磷脂中花生四烯酸和二十二碳六烯酸含量沈晓京，赖炳森，汪聪慧，董风霞（解放军北京医学高等专科学校生化教研室，北京１０００７１；北京公安部第二研究所仪器分析室，１０００３８）磷脂中不饱和脂肪酸是体内多烯酯酸的重要来源，尤其是机体正...The fatty acid(FA)compositions of yolk phospholipid(PL)obtained by supercritical fluid CO2 extraction(SFE-CO2)were analyzed by GC/MS。The method of internal standardquantitation of arachidonic acid(AA,C20:4 ) and docosahexaenoic acid(DHA,C22:6)in yolk PLwas established. It includes the following steps:SFE-CO2 extraction of fresh egg yolk powder to getyolk PL; methylation of FAs in yolk PL and checking the esterification degree with TLC;theidentification of FA compositions in the yolk PL by G C/MS,including the matching of their massspectra with NIST library and of retention times with twelve FA methyl ester standards;the making-up of standard curve using internal standards of C20: 0 and C24:1.A linear relationship was obtainedin the concentration rage of 25～150 ng·μl-1 and the correlation coefficients(Y)of AA and DHAwere 6.9982 and 0.9983 respectively.The contents of AA and DHA were 6.72%and 2.25%respectively.

关 键 词：蛋黄磷脂 花生四烯酸 DHA 测定 GC/MS 

分 类 号：R977.6[医药卫生—药品] R914.4[医药卫生—药学]