用正交设计法优选老鹰茶总黄酮的提取工艺  

The optimization of the extracting method of total flavonoids from Litse a coreana through orthogonal test

作　　者：程文明[1] 王小华[1] 陆维丽[1] 李俊[1] 

机构地区：[1]安徽医科大学药学院,安徽合肥230032

出　　处：《安徽医药》2005年第10期729-730,共2页Anhui Medical and Pharmaceutical Journal

基　　金：安徽省自然科学基金资助项目(03043001);安徽省人才开发基金资助项目(2004Z030)

摘　　要：目的优选老鹰茶总黄酮提取的最佳工艺。方法以芦丁为对照,AlCl3为显色剂,用紫外分光光度法在410nm处测定总黄酮含量,以总黄酮含量为指标,应用正交实验,优选老鹰茶总黄酮提取的最佳工艺。结果老鹰茶总黄酮的最佳提取工艺为80%乙醇,12倍量,回流提取3次,每次2h。结论紫外分光光度法方便、快捷,可用于老鹰茶总黄酮提取工艺的优选。Aim To optimize the extraction procedure of the total flavonoids from Litsea coreana. Methods The optimized extraction procedure was screened by orthogonal test according to the content of the total flavonoids which was measured through UV spectroscopy at 410 nm using turin as control, AlCl3 as reagent. Results The optimized extraction procedure was as follows： 80% alcohol, 12 times volume of the weight of the dried material, refluxing 2 h for 3 times. Conclusion UV spectroscopy is a fast and convienient method suitable for the control of the extraction of the total flavonoids from Litsea coreana.

关 键 词：老鹰茶 总黄酮 正交试验 提取工艺 紫外分光光度法 最佳提取工艺 总黄酮含量 正交设计法 优选 最佳工艺 

分 类 号：TQ461[化学工程—制药化工]