D-半乳糖衰老模型大鼠肝脏形态学及抗氧化能力变化的研究  

Liver morphology of and anti-oxidation capability in aging rat model induced by D-gal

作　　者：徐辉[1] 魏晓东[1] 欧芹[1] 王景涛[1] 辛红[2] 

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007 [2]天津市人民医院,天津300121

出　　处：《黑龙江医药科学》2005年第5期26-27,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的:观察D-半乳糖致衰大鼠肝脏形态学改变及氧化水平和抗氧化能力的变化。方法:采用D-半乳糖所致衰老模型大鼠,做肝组织HE染色光镜观察,测定C a2+-ATP酶活性,肝线粒体M DA含量、PLA2活性。结果:衰老模型大鼠肝组织形态学发生明显变化,肝脏C a2+-ATP酶活性显著降低(P<0.01),肝线粒体M DA含量、PLA2活性显著升高(P<0.01)。结论:D-半乳糖致衰大鼠可作为研究衰老的模型。Objective： To investigate the liver morphology and the oxidation and anti- oxidation capability of aging rat model induced by D-gal. Methods： We used the aging model rat induced by D-gal as research sample, and observed the hepatic tissue HE staining under light microscope. We measured the Ca^2＋- ATPase activity in rat liver and determined the MDA content, PLA2 activity of rat liver mitochondria. Results： The liver morphology was markedly changed and Ca^2＋- ATPase activity was obviously decreased （P 〈0.01 ）and the MDA content and PLA2 activity were markedly increased （P〈0.01）in the aging rat model. Conclusion： The aging rat model induced by D-gal can be used as the model for aging research.

关 键 词：D-半乳糖 衰老模型 形态学 抗氧化能力 大鼠 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学]