无机诱导因子复合性支架材料修复骨缺损的组织学观察  

Histological Observation of Reconstructing Mandible Defect with Prefabricated-mineralized Inducing Bone Tissue Engineering Scaffold Material

作　　者：叶茂昌[1] 周来生[2] 陈柯[3] 胡闻[3] 陈梅梅[1] 李容新[1] 王来平[1] 

机构地区：[1]安徽省立医院干细胞/组织工程研究室,安徽合肥230001 [2]美国波士顿大学分子生物相容学研究室,美国波士顿021182394 [3]安徽省立医院病理科,安徽合肥230001

出　　处：《口腔颌面外科杂志》2005年第3期247-250,共4页Journal of Oral and Maxillofacial Surgery

基　　金：安徽省重点科研项目(03021009);安徽省国际科技合作重点项目(04088009)

摘　　要：目的:应用无机诱导因子复合性支架材料修复山羊的下颌骨角部大型箱状缺损,研究该材料的成骨性能,探讨其在骨组织工程中的应用价值。方法:山羊15只,按4、8、12周3个时间点分为3组,于双侧下颌角手术制备30mm×25mm×10mm的大型箱状缺损,采用自身配对设计,左侧置入支架材料(A组),右侧空白对照(B组)。术后4、8、12周分别处死动物,取缺损修复区和材料—骨界面标本,观察组织动态学。结果:!4～12周A组有进行性成骨、钙化、骨髓形成的过程,而B组骨缺损内为未充满的纤维结缔组织,下缘有不成熟的软骨细胞附着。结论:支架材料有相当良好的生物活性;修复骨缺损显示优良的修复效果;具有临床应用的可行性。Objective： To study the osteogenesis capability of prefabricated-mineralized inducement scaffold material by reconstructing the large-scale chest-like defect in the mandible cornu of the experimental goat, and the application value of the material in the bone tissue engineering. Methods： 15 goats were operated on both sides of mandible cornu to prepare the large-scale chest-like defects of 30 mm×25mm×10mm, with prefabricated-mineralized inducement scaffold material put into the left side（group A）, and blank control on the right side （group B）. These goats were divided into three groups according to the three time points of 4, 8, 12 weeks. After the operation, the animals were executed 4, 8, 12 weeks respectively. The defect restoration areas and the material-bone interface samples were taken to observe tissue dynamics. Results： 4-12 weeks later, bone defects of group A had the process of progressing osteogenesis, calcification and bone marrow formation. The bone defects of group B were fiber connective tissue, whose inferior margins were immature chondrocyte attachment. Conclusion： Prefabricated-mineralized inducement scaffold material, with quite good bioactivity and bone reconstructed ability, has the feasibility to clinical application.

关 键 词：无机诱导因子 支架材料 组织学 组织学观察 骨缺损 下颌骨 

分 类 号：R782[医药卫生—口腔医学]