不同诱导剂对工程菌发酵及重组蛋白表达的影响  

Influence of Various Inducers on Expression of Recombinant Protein by Fermentation

作　　者：卫红飞[1] 万敏[1] 杨世杰[1] 王燕媚[1] 李大鹏[1] 王爱丽[1] 于永利[1] 王丽颖[1] 

机构地区：[1]吉林大学医学部基础医学院,长春130021

出　　处：《中国生物制品学杂志》2005年第6期518-519,525,共3页Chinese Journal of Biologicals

基　　金：国家高技术研究发展计划(编号:2002aa214141)

摘　　要：目的观察不同诱导剂对工程菌发酵及HSP-MUC1重组蛋白表达的影响,从而选取最佳诱导表达条件。方法分别用IPTG和乳糖作为诱导剂,发酵表达HSP-MUCI重组蛋白质,并对重组蛋白质表达量和性状进行分析。结果用IPTG诱导发酵的菌体经普通匀浆法即可将细菌破碎;而用乳糖发酵的菌体经普通匀浆法不能破菌,通过延长发酵时间也能达到与IPTG诱导相同的表达量。结论乳糖可以作为基因工程重组蛋白的理想诱导剂。Objective To study the influence of various inducers on the expression of recombinant HSP-MUC1 protein in E. coli BL21（DE3） by fermentation and optimize the condition for expression.Methods Express recombinant HSP-MUC1 protein by fermentation using IPTG and lactose as inducers respectively, and analyze the expression level and character of HSP-MUC1. Results The bacteria using IPTG as an inducer could be broken by common homogenization. However, the bacteria using lactose as inducer could not be broken by the same method.As the increasing time for expression, the expression level of HSP-MUC1 protein using lactose was comparable to that using IPTG as inducer. Conclusion Lactose might be an ideal inducer for the expression of recombinant protein.

关 键 词：诱导剂 工程菌 重组蛋白 基因表达 细菌发酵 乳糖 基因工程 

分 类 号：Q939.1[生物学—微生物学] Q51