实时荧光定量PCR方法检测人癌组织中RFP蛋白的表达  

Determination of RFP Expression in Human Cancer Tissue by Real-time Fluorescence Quantitative PCR

作　　者：张静敏[1] 金宁一[2] 连海[2] 管国芳[3] 李宵[2] 安汝国[1] 高桥雅英[4] 

机构地区：[1]吉林大学药学院,长春130021 [2]解放军军事医学科学院解放军基因工程重点实验室,长春130062 [3]吉林大学第二医院耳鼻喉科,长春130041 [4]日本名古屋大学病理学实验室

出　　处：《中国生物制品学杂志》2005年第6期520-522,共3页Chinese Journal of Biologicals

摘　　要：目的检测RFP(Ret finger protein)蛋白在人正常组织及人癌组织中的分布。方法采用实时荧光定量PCR方法,以18 S rRNA为内对照,检测RFP在人正常组织及人癌组织中的表达水平。结果RFP表达水平子宫颈鳞状细胞癌组织高于正常子宫颈组织,子宫内膜腺癌组织高于正常子宫内膜组织,胃腺癌组织高于正常胃组织,食管鳞状细胞癌组织高于正常食管组织,而子宫内膜腺癌组织高于子宫颈鳞状细胞癌组织,胃腺癌组织高于食管鳞状细胞癌组织,脑癌组织高于正常脑组织。结论RFP可能成为治疗恶性肿瘤的一个潜在的分子靶点。Objective To study the distribution of ret finger protein（RFP） in normal human and cancer tissues. Methods Determine the expression levels of RFP in normal human and cancer tissues by real-time fluorescence quantitative PCR using 18 S rRNA as an internal standard. Results The expression level of RFP was higher in cervical squamous cell carcinoma tissue than that in normal cervical tissue, higher in endometraial adenocarcinoma tissue than that in normal endometrial tissue, higher in gastric adenocarcinoma tissue than that in normal gastric tissue,higher in esophageal squamous cell carcinoma tissue than that in normal esophageal tissue,higher in endometrial adenocarcinoma tissue than that in cervical squamous cell carcinoma tissue, higher in gastric adenocarcinoma tissue than that in esophageal squamous cell carcinoma tissue,and higher in brain cancer tissue than that in normal brain tissue.Conclusion RFP might be a potential molecular target for the treatment of malignant tumors.

关 键 词：实时荧光定量PCR方法 含量检测 人癌组织 RFP蛋白 基因表达 

分 类 号：R730[医药卫生—肿瘤]